Rnf2 knockdown reduces cell viability and promotes cell cycle arrest in gastric cancer cells

  • Authors:
    • Jingfang Zhang
    • Zhenni Sun
    • Yafei Han
    • Ruyong Yao
    • Lu Yue
    • Yan Xu
    • Jisheng Zhang
  • View Affiliations

  • Published online on: March 17, 2017     https://doi.org/10.3892/ol.2017.5868
  • Pages: 3817-3822
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Abstract

Rnf2 is a fundamental component of the polycomb repressive complex 1and acts as the really interesting new gene finger E3 ligase, which is responsible for histone 2A modification. Previous studies have shown that the ring finger protein 2 (Rnf2) is overexpressed in various types of tumor and has a close association with tumor development. However, few studies have been carried out into the expression and biological function of Rnf2 in gastric cancer cells. The present study measured the expression of Rnf2 in gastric cancer cells and normal epithelial gastric cells. The results demonstrate that Rnf2 is upregulated in gastric cancer cells. In addition, the knockdown of Rnf2 inhibited the cell viability and induced increased G1 phase followed by a substantial reduction of the G2/M phase. The expression levels of p21 and p27 were also significantly elevated by the knockdown of Rnf2. These results provide evidence of the oncogenic function of Rnf2 in gastric cancer, possibly through an inhibition of cellular proliferation and a delay of the G2/M phase. Therefore, Rnf2 may be a novel target for the prognosis and therapy of gastric cancer.
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May-2017
Volume 13 Issue 5

Print ISSN: 1792-1074
Online ISSN:1792-1082

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Spandidos Publications style
Zhang J, Sun Z, Han Y, Yao R, Yue L, Xu Y and Zhang J: Rnf2 knockdown reduces cell viability and promotes cell cycle arrest in gastric cancer cells. Oncol Lett 13: 3817-3822, 2017
APA
Zhang, J., Sun, Z., Han, Y., Yao, R., Yue, L., Xu, Y., & Zhang, J. (2017). Rnf2 knockdown reduces cell viability and promotes cell cycle arrest in gastric cancer cells. Oncology Letters, 13, 3817-3822. https://doi.org/10.3892/ol.2017.5868
MLA
Zhang, J., Sun, Z., Han, Y., Yao, R., Yue, L., Xu, Y., Zhang, J."Rnf2 knockdown reduces cell viability and promotes cell cycle arrest in gastric cancer cells". Oncology Letters 13.5 (2017): 3817-3822.
Chicago
Zhang, J., Sun, Z., Han, Y., Yao, R., Yue, L., Xu, Y., Zhang, J."Rnf2 knockdown reduces cell viability and promotes cell cycle arrest in gastric cancer cells". Oncology Letters 13, no. 5 (2017): 3817-3822. https://doi.org/10.3892/ol.2017.5868