MSK1 downregulation is associated with neuronal and astrocytic apoptosis following subarachnoid hemorrhage in rats

Ning,Bo; Guo,Geng; Liu,Hong; Ning,Lei; Sun,Bao‑Liang; Li,Zhen; Wang,Shuo; Lv,Zheng‑Wen; Fan,Cun‑Dong

doi:10.3892/ol.2017.6496

MSK1 downregulation is associated with neuronal and astrocytic apoptosis following subarachnoid hemorrhage in rats

Authors:
- Bo Ning
- Geng Guo
- Hong Liu
- Lei Ning
- Bao‑Liang Sun
- Zhen Li
- Shuo Wang
- Zheng‑Wen Lv
- Cun‑Dong Fan
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Affiliations: Department of Neurosurgery, Guangzhou Red Cross Hospital, Jinan University, Guangzhou, Guangdong 510220, P.R. China, Department of Neurosurgery, Beijing Tiantan Hospital, Capital Medical University, Beijing 100050, P.R. China, Department of Scientific Research, Taian Central Hospital, Taian, Shandong 271000, P.R. China, Department of Medical Records, Affiliated Hospital of Taishan Medical University, Taian, Shandong 271000, P.R. China, Key Lab of Cerebral Microcirculation in Universities of Shandong, Taishan Medical University, Taian, Shandong 271000, P.R. China, Department of Neurosurgery, Taian Central Hospital, Taian, Shandong 271000, P.R. China
Published online on: June 30, 2017 https://doi.org/10.3892/ol.2017.6496
Pages: 2940-2946
Copyright: © Ning et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

MSK (mitogen‑ and stress‑activated protein kinase) proteins are a family of mitogen‑activated protein kinases. MSKs represent a novel type of pro‑survival genes, potentially enhancing the phosphorylation of Bcl2‑associated agonist of cell death. However, MSK's function and expression are poorly understood in the central nervous system. In the present study, a subarachnoid hemorrhage (SAH) model was established in SD rats and the expression of MSK1 in the brain subsequent to experimental SAH was investigated. In response to SAH, MSK1 mRNA and protein levels gradually declined, reaching the lowest point at 3 days, and increased thereafter. The expression of active caspase‑3 was negatively correlated with MSK1 level. Colocalization and correlating changes in expression of MSK1 and active caspase‑3 at neurons and astrocytes indicated that MSK1 downregulation may contribute to SAH‑induced apoptosis, validating that MSK1 may be involved in the pathophysiology of the brain cortex subsequent to SAH.

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