Open Access

Characteristics of primary side population cervical cancer cells

  • Authors:
    • Zhen‑Tong Wei
    • Xiao‑Wei Yu
    • Jia‑Xue He
    • Yan Liu
    • Song‑Ling Zhang
  • View Affiliations

  • Published online on: July 18, 2017     https://doi.org/10.3892/ol.2017.6606
  • Pages: 3536-3544
  • Copyright: © Wei et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

The aim of the present study was to identify and characterize side population (SP) cells in primary cervical cancer. A primary culture was successfully established, and the SP cells were isolated via fluorescence‑activated cell sorting. Subsequently, in vitro analysis of clonogenic capacity by soft agar assay and in vivo analysis of tumorigenicity were performed. The isolated SP cells accounted for ~4.73% of the total primary culture cells. The SP cells had a decreased prolife­ration rate and an increased distribution in G0/G1 compared with non‑SP (NSP) cells. Following isolation, SP cells exhibited increased proliferative and self‑renewal potency compared with NSP cells. Furthermore, significant ATP binding cassette subfamily G member 2 (ABCG2) expression was detected in SP cells but not in NSP cells. The tumor formation rate of SP cells was longer, and the tumor size and tumor formation rate of SP cells were increased in non‑obese diabetic/severe combined immunodeficiency mice. In conclusion, the present study demonstrated that SP cells can be isolated from primary cervical cancer cell culture, and SP cells are enriched with stem cell‑like cells that have a high capacity for colony formation and tumorigenesis.
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September-2017
Volume 14 Issue 3

Print ISSN: 1792-1074
Online ISSN:1792-1082

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Copy and paste a formatted citation
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Spandidos Publications style
Wei ZT, Yu XW, He JX, Liu Y and Zhang SL: Characteristics of primary side population cervical cancer cells. Oncol Lett 14: 3536-3544, 2017.
APA
Wei, Z., Yu, X., He, J., Liu, Y., & Zhang, S. (2017). Characteristics of primary side population cervical cancer cells. Oncology Letters, 14, 3536-3544. https://doi.org/10.3892/ol.2017.6606
MLA
Wei, Z., Yu, X., He, J., Liu, Y., Zhang, S."Characteristics of primary side population cervical cancer cells". Oncology Letters 14.3 (2017): 3536-3544.
Chicago
Wei, Z., Yu, X., He, J., Liu, Y., Zhang, S."Characteristics of primary side population cervical cancer cells". Oncology Letters 14, no. 3 (2017): 3536-3544. https://doi.org/10.3892/ol.2017.6606