iTRAQ‑coupled 2D LC/MS‑MS analysis of CXCR7‑transfected papillary thyroid carcinoma cells: A new insight into CXCR7 regulation of papillary thyroid carcinoma progression and identification of potential biomarkers

  • Authors:
    • Hengwei Zhang
    • Lei Yang
    • Zhangyi Liu
    • Chenxi Liu
    • Xuyong Teng
    • Lei Zhang
    • Bo Yin
    • Zhen Liu
  • View Affiliations

  • Published online on: July 15, 2017     https://doi.org/10.3892/ol.2017.6574
  • Pages: 3734-3740
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Abstract

Previous studies have demonstrated that C‑X‑C chemokine receptor type 7 (CXCR7) regulates papillary thyroid carcinoma (PTC) growth and metastasis; however, the molecular mechanisms underlying this regulation remain unclear. In the present study, the protein expression profiles of the PTC cell line GLAG‑66 and GLAG‑66 cells stably transfected with CXCR7 cDNA were analyzed and compared using isobaric tag for relative and absolute quantification‑coupled two‑dimensional liquid chromatography‑tandem mass spectrometry. In total, 2,983 proteins were quantified and 130 proteins were identified to be differentially expressed, of which 87 were significantly upregulated and 43 were significantly downregulated. Gene Ontology enrichment analysis revealed that the differentially expressed proteins were primarily enriched in a number of biological processes, including metabolism‑related processes, cellular component organization, transport, cellular development process and the immune response. The differentially expressed proteins identified included fibronectin 1, basigin, periplakin and serpin family B member 5, all of which are associated with cellular junctions and cancer progression. In addition, transgelin‑2 and AHNAK nucleoprotein 2 were identified as potential novel biomarkers for the prognosis and treatment of PTC.
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September-2017
Volume 14 Issue 3

Print ISSN: 1792-1074
Online ISSN:1792-1082

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Spandidos Publications style
Zhang H, Yang L, Liu Z, Liu C, Teng X, Zhang L, Yin B and Liu Z: iTRAQ‑coupled 2D LC/MS‑MS analysis of CXCR7‑transfected papillary thyroid carcinoma cells: A new insight into CXCR7 regulation of papillary thyroid carcinoma progression and identification of potential biomarkers. Oncol Lett 14: 3734-3740, 2017.
APA
Zhang, H., Yang, L., Liu, Z., Liu, C., Teng, X., Zhang, L. ... Liu, Z. (2017). iTRAQ‑coupled 2D LC/MS‑MS analysis of CXCR7‑transfected papillary thyroid carcinoma cells: A new insight into CXCR7 regulation of papillary thyroid carcinoma progression and identification of potential biomarkers. Oncology Letters, 14, 3734-3740. https://doi.org/10.3892/ol.2017.6574
MLA
Zhang, H., Yang, L., Liu, Z., Liu, C., Teng, X., Zhang, L., Yin, B., Liu, Z."iTRAQ‑coupled 2D LC/MS‑MS analysis of CXCR7‑transfected papillary thyroid carcinoma cells: A new insight into CXCR7 regulation of papillary thyroid carcinoma progression and identification of potential biomarkers". Oncology Letters 14.3 (2017): 3734-3740.
Chicago
Zhang, H., Yang, L., Liu, Z., Liu, C., Teng, X., Zhang, L., Yin, B., Liu, Z."iTRAQ‑coupled 2D LC/MS‑MS analysis of CXCR7‑transfected papillary thyroid carcinoma cells: A new insight into CXCR7 regulation of papillary thyroid carcinoma progression and identification of potential biomarkers". Oncology Letters 14, no. 3 (2017): 3734-3740. https://doi.org/10.3892/ol.2017.6574