Impacts of survivin and caspase‑3 on apoptosis and angiogenesis in oral cancer

Li,Shuxia; Yang,Yanqi; Ding,Yanping; Tang,Xiaofei; Sun,Zheng

doi:10.3892/ol.2017.6626

Impacts of survivin and caspase‑3 on apoptosis and angiogenesis in oral cancer

Authors:
- Shuxia Li
- Yanqi Yang
- Yanping Ding
- Xiaofei Tang
- Zheng Sun
View Affiliations

Affiliations: Department of Oral Medicine, Beijing Stomatological Hospital of China, Capital Medical University, Beijing 100050, P.R. China, Department of Orthodontics, School of Dentistry, Hong Kong University, Hong Kong 999077, P.R. China, Institute of Stomatology, Capital Medical University, Beijing 100050, P.R. China
Published online on: July 20, 2017 https://doi.org/10.3892/ol.2017.6626
Pages: 3774-3779

Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )

Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )

Cited By (CrossRef): 0 citations Loading Articles...

Abstract

The present study aimed to investigate the impact of survivin and caspase‑3 on apoptosis and angiogenesis in oral cancer. A total of 16 oral leukoplakia cases accompanied by low‑moderate epithelial dysplasia, 12 cases of oral leukoplakia accompanied by severe epithelial dysplasia, 17 cases of high‑moderate differentiated oral squamous cell carcinoma and 10 cases of normal oral mucosa were selected. Immunohistochemistry was used to detect the expression levels of survivin, caspase‑3, and caspase inhibitor factor VIII in lesions from each group. Terminal deoxynucleotidyl transferase 2'‑deoxyuridine, 5'‑triphosphate nick end labeling was performed to detect the apoptotic index of oral leukoplakia and cancer tissue. Immunohistochemistry revealed increased expression levels of survivin in oral cancer tissues, as compared with the normal mucosa, whereas the expression of Caspase‑3 was decreased during malignant transformation. Microvascular density (MVD) was increased from 28.49±11.87 strips/mm2 (mean ± standard deviation, normal control group) to 91.98±40.20 strips/mm2 (oral cancer group). Therefore, survivin may serve an important role in oral cancer, as its expression was increased in association with a downward trend in caspase‑3 expression and apoptotic index, whereas MVD was significantly increased.

View Figures

View References

1	Sugerman PB, Joseph BK and Savage NW: Review article: The role of oncogenes, tumour suppressor genes and growth factors in oral squamous cell carcinoma: A case of apoptosis versus proliferation. Oral Dis. 1:172–188. 1995. View Article : Google Scholar : PubMed/NCBI
2	Nassar A, Lawson D, Cotsonis G and Cohen C: Survivin and caspase-3 expression in breast cancer: Correlation with prognostic parameters, proliferation, angiogenesis, and outcome. Appl Immunohistochem Mol Morphol. 16:113–120. 2008. View Article : Google Scholar : PubMed/NCBI
3	Lin CY, Hung HC, Kuo RC, Chiang CP and Kuo MY: Survivin expression predicts poorer prognosis in patients with areca quid chewing-related oral squamous cell carcinoma in Taiwan. Oral Oncol. 41:645–654. 2005. View Article : Google Scholar : PubMed/NCBI
4	Lauxen IS, Oliverira MG, Rados PV, Lingen MW, Nör JE and Sant'ana Filho M: Immunoprofiling of oral squamous cell carcinomas reveals high p63 and survivin expression. Oral Dis. 20:e76–e80. 2014. View Article : Google Scholar : PubMed/NCBI
5	Cai Y, Ma W, Huang X, Cao L, Li H, Jiang Y, Lu N and Yin Y: Effect of survivin on tumor growth of colorectal cancer in vivo. Int J Clin Exp Pathol. 8:13267–13272. 2015.PubMed/NCBI
6	Mishra R, Palve V, Kannan S, Pawar S and Teni T: High expression of survivin and its splice variants survivin ΔEx3 and survivin 2 B in oral cancers. Oral Surg Oral Med Oral Pathol Oral Radiol. 120:497–507. 2015. View Article : Google Scholar : PubMed/NCBI
7	Altieri DC: The molecular basis and potential role of survivin in cancer diagnosis and therapy. Trends Mol Med. 7:542–547. 2001. View Article : Google Scholar : PubMed/NCBI
8	Axéll T, Pindborg JJ, Smith CJ and van der Waal I: Oral white lesions with special reference to precancerous and tobacco-related lesions: Conclusions of an international symposium held in Uppsala, Sweden, May 18–21 1994. International collaborative group on oral white lesions. J Oral Pathol Med. 25:49–54. 1996. View Article : Google Scholar : PubMed/NCBI
9	Deng J, Kloosterbooer F, Xia W and Hung MC: The NH(2)-terminal and conserved region 2 domains of adenovirus E1A mediate two distinct mechanisms of tumor suppression. Cancer Res. 62:346–350. 2002.PubMed/NCBI
10	Rowley H, Sherrington P, Helliwell TR, Kinsella A and Jones AS: p53 expression and p53 gene mutation in oral cancer and dysplasia. Otolaryngol Head Neck Surg. 118:115–123. 1998. View Article : Google Scholar : PubMed/NCBI
11	Duffy MJ, O'Donovan N, Brennan DJ, Gallagher WM and Ryan BM: Survivin: A promising tumor biomarker. Cancer Lett. 249:49–60. 2007. View Article : Google Scholar : PubMed/NCBI
12	Lo Muzio L, Pannone G, Leonardi R, Staibano S, Mignogna MD, De Rosa G, Kudo Y, Takata T and Altieri DC: Survivin, a potential early predictor of tumor progression in the oral mucosa. J Dent Res. 82:923–928. 2003. View Article : Google Scholar : PubMed/NCBI
13	Poomsawat S, Punyasingh J and Vejchapipat P: Overexpression of survivin and caspase 3 in oral carcinogenesis. Appl Immunohistochem Mol Morphol. 22:65–71. 2014. View Article : Google Scholar : PubMed/NCBI
14	Grossman D, McNiff JM, Li F and Altieri DC: Expression of the apoptosis inhibitor, survivin, in nonmelanoma skin cancer and gene targeting in a keratinoyte cell line. J Lab Invest. 79:1121–1126. 1999.
15	Li SX, Chai L, Cai ZG, Jin LJ, Chen Y, Wu HR and Sun Z: Expression of survivin and caspase 3 in oral squamous cell carcinoma and peritumoral tissue. Asian Pac J Cancer Prev. 13:5027–5031. 2012. View Article : Google Scholar : PubMed/NCBI
16	Kania J, Konturek SJ, Marlicz K, Hahn EG and Konturek PC: Expression of survivin and caspase-3 in gastric cancer. Dig Dis Sci. 48:266–271. 2003. View Article : Google Scholar : PubMed/NCBI
17	Wang ZM, Zhao ZG, Guan XM, Liu F and Zhang LP: Expression of Survivin mRNA and Caspase-3 mRNA and their correlation in tongue squamous cell carcinoma. Shanghai Kou Qiang Yi Xue. 16:582–586. 2007.(In Chinese). PubMed/NCBI
18	Xu JH, Wang AX, Huang HZ, Wang JG, Pan CB and Zhang B: Survivin shRNA induces caspase-3-dependent apoptosis and enhances cisplatin sensitivity in squamous cell carcinoma of the tongue. Oncol Res. 18:377–385. 2010. View Article : Google Scholar : PubMed/NCBI
19	Holdenrieder S and Stieber P: Circulating apoptotic markers in the management of non-small cell lung cancer. Cancer Biomark. 6:197–210. 2010. View Article : Google Scholar : PubMed/NCBI
20	Zhang JY, Casiano CA, Peng XX, Koziol JA, Chan EK and Tan EM: Enhancement of antibody detection in cancer using panel of recombinant tumor-associated antigens. Cancer Epidemiol Biomarkers Prev. 12:136–143. 2003.PubMed/NCBI
21	Srinivasprasad V, Dineshshankar J, Sathiyajeeva J, Karthikeyan M, Sunitha J and Ragunathan R: Liaison between micro-organisms and oral cancer. J Pharm Bioallied Sci. 7:(Suppl 2). S354–S360. 2015. View Article : Google Scholar : PubMed/NCBI
22	Hills SA and Diffley JF: DNA replication and oncogene-induced replicative stress. Curr Biol. 24:R435–R444. 2014. View Article : Google Scholar : PubMed/NCBI
23	Wang S, Xu J and Zhang Q: Clinical significance of survivin and vascular endothelial growth factor mRNA detection in the peripheral whole blood of breast cancer patients. Neoplasma. 63:133–140. 2016. View Article : Google Scholar : PubMed/NCBI
24	Gao J, Huang Y, Li M, Zhao H, Zhao Y, Li R, Yan J, Yu Y and Qiao J: Effect of local basic fibroblast growth factor and vascular endothelial growth factor on subcutaneously allotransplanted ovarian tissue in ovariectomized mice. PLoS One. 10:e01340352015. View Article : Google Scholar : PubMed/NCBI
25	Ji ZJ, Wang JL and Chen L: Inhibition of skin squamous cell carcinoma proliferation and promote apoptosis by dual silencing of NET-1 and survivin. Oncol Rep. 34:811–822. 2015.PubMed/NCBI
26	Coma S, Noe V, Lavarino C, Adán J, Rivas M, López-Matas M, Pagan R, Mitjans F, Vilaró S, Piulats J and Ciudad CJ: Use of siRNAs an d antisense oligonucleotides against survivin RNA to inhibit steps leading to tumor angiogenesis. Oligonucleotides. 14:100–113. 2004. View Article : Google Scholar : PubMed/NCBI
27	Tu SP, Cui JT, Liston P, Huajiang X, Xu R, Lin MC, Zhu YB, Zou B, Ng SS, Jiang SH, et al: Gene therapy for colon cancer by adeno-associated viral vector-mediated transfer of surviving Cys84Ala mutant. Gastroenterology. 128:361–375. 2005. View Article : Google Scholar : PubMed/NCBI
28	Cosgrave N, Hill AD and Young LS: Growth factor-dependent regulation of survivin by c-myc in human breast cancer. J Mol Endocrinol. 37:377–390. 2006. View Article : Google Scholar : PubMed/NCBI