Open Access

Ouabain targets the Na+/K+‑ATPase α3 isoform to inhibit cancer cell proliferation and induce apoptosis

  • Authors:
    • Yijun Xiao
    • Chen Meng
    • Jie Lin
    • Chaoqun Huang
    • Xiuli Zhang
    • Yanyu Long
    • Yide Huang
    • Yao Lin
  • View Affiliations

  • Published online on: September 26, 2017     https://doi.org/10.3892/ol.2017.7070
  • Pages:6678-6684
  • Copyright: © Xiao et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Ouabain has been used for the treatment of heart failure and atrial fibrillation. Its potential anticancer effect has also attracted great interest. The aim of the present study was to evaluate the anticancer effect of ouabain and investigate its molecular target. The effects of ouabain on the viability of and induction of cellular death on OS‑RC‑2 renal cancer cells were examined using the MTT assay and acridine orange/ethidium bromide staining. The levels of Ca2+ and reactive oxygen species were determined using Fura‑3‑acetoxymethyl ester and dichloro‑dihydro‑fluorescein diacetate probes, respectively. Apoptosis was examined using annexin V‑fluorescein isothiocyanate/propidium iodide staining and western blotting. The expression profile of the different Na+/K+‑ATPase (NKA) isoforms in NCI‑H446 small cell lung cancer cells was determined using immunocytochemistry and reverse transcription polymerase chain reaction analysis. In the present study, it was demonstrated that ouabain inhibited cancer cell proliferation and induced apoptosis while no significant difference in the expression of NKA α1 and α3 isoforms was detected following 48 h of ouabain treatment. Furthermore, expression of NKA α3 but not the α1 isoform was associated with ouabain sensitivity. The results of the present study indicated that ouabain targets the NKA α3 isoform, inhibits cancer cell proliferation and induces apoptosis.

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December 2017
Volume 14 Issue 6

Print ISSN: 1792-1074
Online ISSN:1792-1082

2016 Impact Factor: 1.39
Ranked #68/217 Oncology
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APA
Xiao, Y., Meng, C., Lin, J., Huang, C., Zhang, X., Long, Y. ... Lin, Y. (2017). Ouabain targets the Na+/K+‑ATPase α3 isoform to inhibit cancer cell proliferation and induce apoptosis. Oncology Letters, 14, 6678-6684. https://doi.org/10.3892/ol.2017.7070
MLA
Xiao, Y., Meng, C., Lin, J., Huang, C., Zhang, X., Long, Y., Huang, Y., Lin, Y."Ouabain targets the Na+/K+‑ATPase α3 isoform to inhibit cancer cell proliferation and induce apoptosis". Oncology Letters 14.6 (2017): 6678-6684.
Chicago
Xiao, Y., Meng, C., Lin, J., Huang, C., Zhang, X., Long, Y., Huang, Y., Lin, Y."Ouabain targets the Na+/K+‑ATPase α3 isoform to inhibit cancer cell proliferation and induce apoptosis". Oncology Letters 14, no. 6 (2017): 6678-6684. https://doi.org/10.3892/ol.2017.7070