miR‑20b promotes cellular proliferation and migration by directly regulating phosphatase and tensin homolog in prostate cancer

  • Authors:
    • Ju Guo
    • Zewen Xiao
    • Xingwei Yu
    • Runfu Cao
  • View Affiliations

  • Published online on: September 25, 2017     https://doi.org/10.3892/ol.2017.7041
  • Pages:6895-6900
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Abstract

MicroRNAs are small non‑coding RNAs, which are critical regulators of carcinogenesis and tumor progression. Previous studies have identified that microRNA‑20b (miR‑20b) acts as an oncogene in numerous cancers. However, the role of miR‑20b in prostate cancer remains unclear. The present study aimed to investigate the expression of miR‑20b in prostate cancer and to examine whether modulating miR‑20b expression impacts prostate cancer cellular proliferation and migration. It was revealed that miR‑20b was strongly expressed in prostate cancer tissues compared with adjacent normal prostate tissues (P<0.05). Knockdown of miR‑20b expression by miR‑20b inhibitor inhibited VCaP and PC‑3 cell growth and migration. Through bioinformatics analysis, phosphatase and tensin homolog (PTEN) was predicted as a target gene of miR‑20b in prostate cancer cells, which was validated by dual‑luciferase reporter assay and western blot analysis. In addition, restoration of PTEN expression levels did not affect endogenous miR‑20b expression in prostate cancer cells. In conclusion, the present study indicated that miR‑20b promotes cellular proliferation and migration by directly regulating PTEN in prostate cancer.

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December 2017
Volume 14 Issue 6

Print ISSN: 1792-1074
Online ISSN:1792-1082

2016 Impact Factor: 1.39
Ranked #68/217 Oncology
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APA
Guo, J., Xiao, Z., Yu, X., & Cao, R. (2017). miR‑20b promotes cellular proliferation and migration by directly regulating phosphatase and tensin homolog in prostate cancer. Oncology Letters, 14, 6895-6900. https://doi.org/10.3892/ol.2017.7041
MLA
Guo, J., Xiao, Z., Yu, X., Cao, R."miR‑20b promotes cellular proliferation and migration by directly regulating phosphatase and tensin homolog in prostate cancer". Oncology Letters 14.6 (2017): 6895-6900.
Chicago
Guo, J., Xiao, Z., Yu, X., Cao, R."miR‑20b promotes cellular proliferation and migration by directly regulating phosphatase and tensin homolog in prostate cancer". Oncology Letters 14, no. 6 (2017): 6895-6900. https://doi.org/10.3892/ol.2017.7041