Combination of histoculture drug response assay and qPCR as an effective method to screen biomarkers for personalized chemotherapy in esophageal cancer

  • Authors:
    • Bin Wei
    • Jiru Wang
    • Xiaohui Zhang
    • Zhaoye Qian
    • Jingjing Wu
    • Yuan Sun
    • Qin Han
    • Li Wan
    • Jing Zhu
    • Yong Gao
    • Xiaofei Chen
  • View Affiliations

  • Published online on: September 26, 2017     https://doi.org/10.3892/ol.2017.7069
  • Pages:6915-6922
0

Abstract

Personalized chemotherapy with the use of biomarkers helps to maximize clinical efficiency. Therefore, the present study aimed to identify a potential method for identifying biomarkers in esophageal cancer. A total of 49 freshly resected tumor tissues and 72 paraffin‑embedded specimens from patients with esophageal cancer were obtained. mRNA expression levels of ERCC1, BRCA1, TUBB3, FBW7, RRM1, MDM2, TS and TOP1 were measured quantitative reverse transcription polymerase chain reaction (RT‑qPCR). In vitro chemosensitivity to cisplatin, docetaxel, gemcitabine, etoposide, fluorouracil and irinotecan were tested using histoculture drug response assay (HDRA). BRCA1 mRNA levels were positively correlated with resistance to cisplatin (P=0.027) and sensitivity to docetaxel (P=0.002). TS mRNA levels were inversely correlated with fluorouracil sensitivity (P=0.044), and TOP1 mRNA expression was positively correlated with irinotecan sensitivity (P=0.008). In addition, high BRCA1 mRNA levels correlated with decreased median overall survival (mOS; P<0.001) and response rate (RR; P=0.002) in cisplatin‑fluorouracil chemotherapy group and also correlated with increased mOS (P<0.001) and RR (P=0.023) in docetaxel‑fluorouracil chemotherapy group. Overall, these results suggested that HDRA combined with RT‑qPCR may serve as an effective method for screening biomarkers in personalized chemotherapy for esophageal cancer.

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December 2017
Volume 14 Issue 6

Print ISSN: 1792-1074
Online ISSN:1792-1082

2016 Impact Factor: 1.39
Ranked #68/217 Oncology
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APA
Wei, B., Wang, J., Zhang, X., Qian, Z., Wu, J., Sun, Y. ... Chen, X. (2017). Combination of histoculture drug response assay and qPCR as an effective method to screen biomarkers for personalized chemotherapy in esophageal cancer. Oncology Letters, 14, 6915-6922. https://doi.org/10.3892/ol.2017.7069
MLA
Wei, B., Wang, J., Zhang, X., Qian, Z., Wu, J., Sun, Y., Han, Q., Wan, L., Zhu, J., Gao, Y., Chen, X."Combination of histoculture drug response assay and qPCR as an effective method to screen biomarkers for personalized chemotherapy in esophageal cancer". Oncology Letters 14.6 (2017): 6915-6922.
Chicago
Wei, B., Wang, J., Zhang, X., Qian, Z., Wu, J., Sun, Y., Han, Q., Wan, L., Zhu, J., Gao, Y., Chen, X."Combination of histoculture drug response assay and qPCR as an effective method to screen biomarkers for personalized chemotherapy in esophageal cancer". Oncology Letters 14, no. 6 (2017): 6915-6922. https://doi.org/10.3892/ol.2017.7069