Open Access

Melanoma differentiation‑associated gene‑7 suppresses human gastric cancer cell invasion and migration

  • Authors:
    • Li Xu
    • Jinyan Chen
    • Wei Lin
    • Jinkun Chen
    • Zhiwei Chen
  • View Affiliations

  • Published online on: September 27, 2017     https://doi.org/10.3892/ol.2017.7086
  • Pages:7139-7144
  • Copyright: © Xu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Gastric cancer is one of the most common types of cancer in the world. Patients with gastric cancer often respond poorly to conventional chemotherapies, therefore more comprehensive therapy is required. Melanoma differentiation‑associated gene‑7 (MDA‑7), also termed interleukin‑24, is a potent tumor suppressor gene. Numerous studies have demonstrated that MDA‑7 suppresses the growth and induces the apoptosis of cancer cells. In the present study, the MDA‑7 gene was transfected into human gastric cancer AGS cells using adenovirus. Transwell and wound healing assays were performed to evaluate AGS cell invasion and migration, respectively. Western blotting was used to detect the expression of epithelial (E)‑cadherin, cluster of differentiation (CD)44 and matrix metalloproteinase (MMP)‑2 and MMP‑9 proteins. A recombinant virus package was successfully constructed, and it was verified using western blotting that exogenous MDA‑7 was highly expressed in the AGS cells. MDA‑7 overexpression inhibited invasion and migration, decreased CD44, MMP‑2 and MMP‑9 expression, and increased epithelial (E‑)cadherin expression in the AGS cells. Results of the present study revealed that MDA‑7 inhibits gastric cancer invasion and metastasis by inhibiting CD44, MMP‑2 and MMP‑9 expression and by promoting E‑cadherin expression.

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December 2017
Volume 14 Issue 6

Print ISSN: 1792-1074
Online ISSN:1792-1082

2016 Impact Factor: 1.39
Ranked #68/217 Oncology
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APA
Xu, L., Chen, J., Lin, W., Chen, J., & Chen, Z. (2017). Melanoma differentiation‑associated gene‑7 suppresses human gastric cancer cell invasion and migration. Oncology Letters, 14, 7139-7144. https://doi.org/10.3892/ol.2017.7086
MLA
Xu, L., Chen, J., Lin, W., Chen, J., Chen, Z."Melanoma differentiation‑associated gene‑7 suppresses human gastric cancer cell invasion and migration". Oncology Letters 14.6 (2017): 7139-7144.
Chicago
Xu, L., Chen, J., Lin, W., Chen, J., Chen, Z."Melanoma differentiation‑associated gene‑7 suppresses human gastric cancer cell invasion and migration". Oncology Letters 14, no. 6 (2017): 7139-7144. https://doi.org/10.3892/ol.2017.7086