Open Access

Regulation of autophagy inhibition and inflammatory response in glioma by Wnt signaling pathway

  • Authors:
    • Tongxin Wang
    • Zhixia Chen
    • Wei Zhang
  • View Affiliations

  • Published online on: September 29, 2017     https://doi.org/10.3892/ol.2017.7103
  • Pages:7197-7200
  • Copyright: © Wang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

The objective of this study was to investigate the mechanism of the function of Wnt signaling pathway in regulating autophagy and inflammatory response in glioma cells. Human brain glioma cells U118 were selected and divided into three groups: i) the Wnt signaling inhibitor IWR-1 group (the observation group); ii) the PBS negative control group (the PBS group) and iii) the blank control group. After 24 h culture, Wnt5a/β-catenin protein, autophagy marker, microtubule-associated-proteins-1A1B-light-chain-3C (LC-3) II and Beclin I, and inflammatory factors IL-6 and TNF-α protein expression levels were evaluated using western blotting. Compared with both control groups, Wnt5a/β-catenin, IL-6 and TNF-α protein expression levels were significantly lower, and LC-3II and Beclin I protein expression levels were significantly higher in the observation group. In conclusion, Wnt5a/β-catenin signaling pathway regulates autophagy and inflammatory response of glioma cells.

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Journal Cover

December 2017
Volume 14 Issue 6

Print ISSN: 1792-1074
Online ISSN:1792-1082

2016 Impact Factor: 1.39
Ranked #68/217 Oncology
(total number of cites)

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APA
Wang, T., Chen, Z., & Zhang, W. (2017). Regulation of autophagy inhibition and inflammatory response in glioma by Wnt signaling pathway. Oncology Letters, 14, 7197-7200. https://doi.org/10.3892/ol.2017.7103
MLA
Wang, T., Chen, Z., Zhang, W."Regulation of autophagy inhibition and inflammatory response in glioma by Wnt signaling pathway". Oncology Letters 14.6 (2017): 7197-7200.
Chicago
Wang, T., Chen, Z., Zhang, W."Regulation of autophagy inhibition and inflammatory response in glioma by Wnt signaling pathway". Oncology Letters 14, no. 6 (2017): 7197-7200. https://doi.org/10.3892/ol.2017.7103