Open Access

Effect of fraxetin on proliferation and apoptosis in breast cancer cells

  • Authors:
    • Guodong Liu
    • Zhenfang Liu
    • Yuexiang Yan
    • Haiyan Wang
  • View Affiliations

  • Published online on: October 5, 2017     https://doi.org/10.3892/ol.2017.7143
  • Pages:7374-7378
  • Copyright: © Liu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

The aim of the present study was to examine the effect of fraxetin on proliferation and apoptosis in the MCF-7 breast cancer cell line. Cell proliferation was measused using an MTT assay and 4’,6-diamidino-2-phenylindole (DAPI) staining was used to determine shrinkage and condensation. RT-PCR was used to examine the expression of factor‑associated suicide (Fas) and Fas ligand (FasL) mRNA, and western blot analysis was used to examine Bax and Bcl-2 protein. MTT showed that the proliferation of MCF-7 cells was significantly inhibited by fraxetin in a dose-dependent manner. Fraxetin also induced significant morphological changes of MCF-7 cells, suggestive of apoptosis, whereas DAPI staining showed that fraxetin caused cell shrinkage and chromatin condensation. RT-PCR showed that the expression of Fas and FasL mRNA was upregulated by fraxetin and the western blot analysis revealed that Bax was upregulated and Bcl-2 was downregulated. In conclusion, fraxetin can inhibit the proliferation of MCF-7 cells, induce apoptosis, upregulate Fas, FasL and Bax, and downregulate Bcl-2 to induce apoptosis. These results support the potential therapeutic role for fraxetin in breast cancer.

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December 2017
Volume 14 Issue 6

Print ISSN: 1792-1074
Online ISSN:1792-1082

2016 Impact Factor: 1.39
Ranked #68/217 Oncology
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APA
Liu, G., Liu, Z., Yan, Y., & Wang, H. (2017). Effect of fraxetin on proliferation and apoptosis in breast cancer cells. Oncology Letters, 14, 7374-7378. https://doi.org/10.3892/ol.2017.7143
MLA
Liu, G., Liu, Z., Yan, Y., Wang, H."Effect of fraxetin on proliferation and apoptosis in breast cancer cells". Oncology Letters 14.6 (2017): 7374-7378.
Chicago
Liu, G., Liu, Z., Yan, Y., Wang, H."Effect of fraxetin on proliferation and apoptosis in breast cancer cells". Oncology Letters 14, no. 6 (2017): 7374-7378. https://doi.org/10.3892/ol.2017.7143