TSLP promotes angiogenesis of human umbilical vein endothelial cells by strengthening the crosstalk between cervical cancer cells and eosinophils

  • Authors:
    • Bing Zhang
    • Chun‑Yan Wei
    • Kai‑Kai Chang
    • Jia‑Jun Yu
    • Wen‑Jie Zhou
    • Hui‑Li Yang
    • Jun Shao
    • Jin‑Jin Yu
    • Ming‑Qing Li
    • Feng Xie
  • View Affiliations

  • Published online on: October 3, 2017     https://doi.org/10.3892/ol.2017.7121
  • Pages:7483-7488
0

Abstract

Our previous study demonstrated that thymic stromal lymphopoietin (TSLP) secreted by cervical cancer cells promotes angiogenesis and recruitment, and regulates the function of eosinophils (EOS). However, the function of TSLP in the crosstalk between EOS and vascular endothelial cells in cancer lesions remains unknown. The aim of the present study was to investigate the effect of EOS caused by TSLP in in vitro angiogenesis of human umbilical vein endothelial cells (HUVECs). The results of the present study revealed that recombinant human TSLP protein (rhTSLP) increased the secretion of vascular endothelial growth factor (VEGF), but not fibroblast growth factors, in HL‑60‑eosinophils (HL‑60E). Compared with cervical cancer cells (HeLa or CasKi cells) or HL‑60E alone, there were increased levels of interleukin (IL)‑8 and VEGF in the co‑culture system between cervical cancer cells, and HL‑60E cells. This effect was strengthened by rhTSLP, but inhibited by inhibiting the TSLP signal with anti‑human TSLP or TSLP receptor neutralizing antibodies. The results of the tube formation assays revealed that treatment with the supernatant from cervical cancer cells and/or HL‑60E resulted in an increase in angiogenesis in HUVECs, which could be decreased by TSLP or TSLPR inhibitors. The results of the present study suggested that TSLP derived of cervical cancer cells may indirectly stimulate angiogenesis of HUVECs, by upregulating IL‑8 and VEGF production, in a co‑culture model between cervical cancer cells and EOS, therefore promoting the development of cervical cancer.

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December 2017
Volume 14 Issue 6

Print ISSN: 1792-1074
Online ISSN:1792-1082

2016 Impact Factor: 1.39
Ranked #68/217 Oncology
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APA
Zhang, B., Wei, C., Chang, K., Yu, J., Zhou, W., Yang, H. ... Xie, F. (2017). TSLP promotes angiogenesis of human umbilical vein endothelial cells by strengthening the crosstalk between cervical cancer cells and eosinophils. Oncology Letters, 14, 7483-7488. https://doi.org/10.3892/ol.2017.7121
MLA
Zhang, B., Wei, C., Chang, K., Yu, J., Zhou, W., Yang, H., Shao, J., Yu, J., Li, M., Xie, F."TSLP promotes angiogenesis of human umbilical vein endothelial cells by strengthening the crosstalk between cervical cancer cells and eosinophils". Oncology Letters 14.6 (2017): 7483-7488.
Chicago
Zhang, B., Wei, C., Chang, K., Yu, J., Zhou, W., Yang, H., Shao, J., Yu, J., Li, M., Xie, F."TSLP promotes angiogenesis of human umbilical vein endothelial cells by strengthening the crosstalk between cervical cancer cells and eosinophils". Oncology Letters 14, no. 6 (2017): 7483-7488. https://doi.org/10.3892/ol.2017.7121