Silibinin inhibits the migration and invasion of human gastric cancer SGC7901 cells by downregulating MMP‑2 and MMP‑9 expression via the p38MAPK signaling pathway

  • Authors:
    • Shuming Lu
    • Zhuqing Zhang
    • Meiru Chen
    • Chunyan Li
    • Lina Liu
    • Yan Li
  • View Affiliations

  • Published online on: September 27, 2017     https://doi.org/10.3892/ol.2017.7080
  • Pages:7577-7582
0

Abstract

The objective of the present study was to observe the effects of silibinin and the p38 mitogen‑activated protein kinase (MAPK) signaling pathway inhibitor SB203580 on the migration and invasion capabilities of SGC7901 cells, and to explore the underlying associated mechanisms. Scratch, Transwell and Matrigel invasion assays were performed to study the effects of silibinin on cell migration and invasion. Western blot analysis was used to determine the expression levels of p38MAPK, phosphorylated (p‑)p38MAPK, matrix metalloproteinase (MMP)‑2 and MMP‑9. At the genomic level, quantitative polymerase chain reaction was performed to evaluate the expression levels of MMP‑2 and MMP‑9. The results of scratch assay indicated that silibinin inhibited the migration capabilities of human gastric cancer SGC7901 cells in a dose‑dependent manner. Additionally, Matrigel invasion and Transwell migration assays revealed that silibinin and SB203580 combined treatment significantly reduced the number of invasive cells. Western blot analysis indicated a reduced phosphorylation of p38MAPK without marked changes in p38MAPK expression. In addition, the expression of MMP‑2 and MMP‑9 significantly decreased in the presence of silibinin, SB203580, and the combination of silibinin and SB203580. In summary, silibinin decreased the invasion and migration abilities of SGC7901 cells by downregulating the expression of MMP‑2 and MMP‑9 through inhibiting p38MAPK signaling cascades.

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December 2017
Volume 14 Issue 6

Print ISSN: 1792-1074
Online ISSN:1792-1082

2016 Impact Factor: 1.39
Ranked #68/217 Oncology
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APA
Lu, S., Zhang, Z., Chen, M., Li, C., Liu, L., & Li, Y. (2017). Silibinin inhibits the migration and invasion of human gastric cancer SGC7901 cells by downregulating MMP‑2 and MMP‑9 expression via the p38MAPK signaling pathway. Oncology Letters, 14, 7577-7582. https://doi.org/10.3892/ol.2017.7080
MLA
Lu, S., Zhang, Z., Chen, M., Li, C., Liu, L., Li, Y."Silibinin inhibits the migration and invasion of human gastric cancer SGC7901 cells by downregulating MMP‑2 and MMP‑9 expression via the p38MAPK signaling pathway". Oncology Letters 14.6 (2017): 7577-7582.
Chicago
Lu, S., Zhang, Z., Chen, M., Li, C., Liu, L., Li, Y."Silibinin inhibits the migration and invasion of human gastric cancer SGC7901 cells by downregulating MMP‑2 and MMP‑9 expression via the p38MAPK signaling pathway". Oncology Letters 14, no. 6 (2017): 7577-7582. https://doi.org/10.3892/ol.2017.7080