Open Access

Association between integrin‑linked kinase and hyperthermia in oral squamous cell carcinoma

  • Authors:
    • Jun Zhao
    • Na Liu
    • Xinhe Hao
    • Lin Que
    • Jiyuan Liu
    • Xiufa Tang
  • View Affiliations

  • Published online on: October 19, 2017     https://doi.org/10.3892/ol.2017.7222
  • Pages:7705-7714
  • Copyright: © Zhao et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

The present study aimed to observe the effect of the biological functions of integrin‑linked kinase (ILK) silencing combined with hyperthermia on Tca8113 cells. Lentivirus‑mediated short hairpin RNA (shRNA)‑targeting ILK was transfected into oral squamous cell carcinoma (OSCC) Tca8113 cells and, combined with hyperthermia, several experimental methods were used to detect their biological behavior in vitro. On the basis of in vitro experiments, Tca8113 cells were transplanted into nude mice models, and ILK‑shRNA‑lentivirus was injected into the nude mice transplanted tumor and combined with hyperthermia. Tumor morphology and the associated protein expression changes were determined. Subsequent to ILK silencing combined with hyperthermia, the growth, migration and proliferation of Tca8113 cells were significantly inhibited. Flow cytometry revealed that the cells were blocked in the S phase, and western blot analysis demonstrated that ILK, phosphorylated (p)‑RAC‑alpha serine/threonine‑protein kinase (Akt), p‑glycogen synthase kinase‑3β and p‑heat shock factor 1 protein expression levels were significantly decreased, while apoptosis‑associated protein B‑cell lymphoma‑2‑associated X protein expression and the efficacy of hypothermia were significantly increased. By ILK silencing combined with hyperthermia, a significant therapeutic effect on transplanted tumors was observed in nude mice. Immunohistochemistry revealed the same results as the in vitro experiments. ILK silencing combined with hyperthermia can inhibit the growth, proliferation and migration of Tca8113 cells, promote Tca8113 cell apoptosis, inhibit the phosphatidylinositol‑3‑kinase/Akt signaling pathway and increase hyperthermia sensitivity; the combination therapy exhibits a synergistic sensitizing effect. Therefore, ILK silencing combined with hypothermia may serve as a novel combination therapy strategy against OSCC.

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December 2017
Volume 14 Issue 6

Print ISSN: 1792-1074
Online ISSN:1792-1082

2016 Impact Factor: 1.39
Ranked #68/217 Oncology
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APA
Zhao, J., Liu, N., Hao, X., Que, L., Liu, J., & Tang, X. (2017). Association between integrin‑linked kinase and hyperthermia in oral squamous cell carcinoma. Oncology Letters, 14, 7705-7714. https://doi.org/10.3892/ol.2017.7222
MLA
Zhao, J., Liu, N., Hao, X., Que, L., Liu, J., Tang, X."Association between integrin‑linked kinase and hyperthermia in oral squamous cell carcinoma". Oncology Letters 14.6 (2017): 7705-7714.
Chicago
Zhao, J., Liu, N., Hao, X., Que, L., Liu, J., Tang, X."Association between integrin‑linked kinase and hyperthermia in oral squamous cell carcinoma". Oncology Letters 14, no. 6 (2017): 7705-7714. https://doi.org/10.3892/ol.2017.7222