Overexpression of microRNA‑1470 promotes proliferation and migration, and inhibits senescence of esophageal squamous carcinoma cells
- Li‑Li Mei
- Yun‑Tan Qiu
- Wen‑Jun Wang
- Jie Bai
- Zhi‑Zhou Shi
Published online on: October 16, 2017
Copyright: © Mei et al.
This is an open access article distributed under the terms of Creative Commons Attribution License.
MicroRNA‑1470 (miR‑1470) is overexpressed in esophageal squamous cell carcinoma (ESCC); however, its role and underlying molecular mechanism remain unknown. The aim of the present study was to explore the tumorigenic role and mechanism of miR‑1470 overexpression in ESCC. The expression of miR‑1470 in ESCC tissues and cell lines was detected using human miRNA microarrays and the reverse transcription‑quantitative polymerase chain reaction, respectively. The effects of miR‑1470 on cell proliferation, migration and senescence were determined using a Cell Counting Kit‑8 assay, Transwell migration assay and β‑galactosidase staining kit. Western blotting was used to analyze the expression levels of genes in the apoptosis signaling pathway. An increased expression level of miR‑1470 was observed in ESCC tissues compared with that in paracancerous tissues. Knockdown of miR‑1470 significantly suppressed proliferation, and down‑regulated the cell cycle regulatory gene cyclin E1. It was also revealed that knockdown of miR‑1470 significantly inhibited migration, and decreased the expression levels of matrix metalloproteinase 2 (MMP2), MMP13 and MMP14. Western blotting analysis revealed that knockdown of miR‑1470 induced apoptosis by increasing B‑cell lymphoma 2 (Bcl‑2) expression. The results of the present study suggest that overexpression of miR‑1470 in ESCC promotes cancer cell proliferation by accelerating the cell cycle and inhibiting apoptosis, and also enhances cancer cell migration by upregulating MMPs.