Low risk HPV‑6E6 induces apoptosis in bone marrow‑derived dendritic cells

  • Authors:
    • Lina Sun
    • Lei Zhao
    • Dan Li
    • Shuai Xu
    • Xuexin Hou
    • Zhenjun Li
  • View Affiliations

  • Published online on: November 15, 2017     https://doi.org/10.3892/ol.2017.7417
  • Pages:1157-1162
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Abstract

The present study assessed the subcellular localization and apoptotic potential of low‑risk human papilloma virus 6E6 (HPV‑6E6), expressed in bone marrow‑derived dendritic cells (DCs). DCs were obtained from C57BL/6 mice and transfected with a pGFP‑6E6 plasmid. The subcellular localization of E6 was determined by tracing green fluorescent protein (GFP) using fluorescence microscopy. Apoptosis was assessed by staining nuclei with DAPI and performing an apoptosis‑based flow cytometry assay. The co‑localization of E6 and p53 was also investigated using confocal microscopy. In addition, the expression of apoptosis‑associated proteins was analyzed using immunoblotting. The results of the present study demonstrated that low‑risk HPV‑6E6 is predominantly localized in the cytoplasm of DCs. Furthermore, p53 was upregulated in DCs transfected with pGFP‑6E6 and co‑localized with GFP‑6E6 in the cytoplasm. DCs transfected with a control pGFP plasmid did not undergo apoptosis, whereas cells transfected with pGFP‑6E6 did, as indicated by the presence of cell debris and condensation of the nuclei. Furthermore, the expression of apoptosis‑associated proteins, including p53, BCL2 associated X apoptosis regulator (Bax), BCL2 homologous antagonist/killer (Bak) and cytochrome c, were significantly higher in DCs expressing low‑risk E6 than in control cells. Therefore, the current study demonstrated that low‑risk HPV‑6E6 is predominantly located in the cytoplasm of DCs and induces apoptosis. This may be an important mechanism that explains why low‑risk HPV is unable to induce malignant transformation.

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January 2018
Volume 15 Issue 1

Print ISSN: 1792-1074
Online ISSN:1792-1082

2016 Impact Factor: 1.39
Ranked #68/217 Oncology
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APA
Sun, L., Zhao, L., Li, D., Xu, S., Hou, X., & Li, Z. (2018). Low risk HPV‑6E6 induces apoptosis in bone marrow‑derived dendritic cells. Oncology Letters, 15, 1157-1162. https://doi.org/10.3892/ol.2017.7417
MLA
Sun, L., Zhao, L., Li, D., Xu, S., Hou, X., Li, Z."Low risk HPV‑6E6 induces apoptosis in bone marrow‑derived dendritic cells". Oncology Letters 15.1 (2018): 1157-1162.
Chicago
Sun, L., Zhao, L., Li, D., Xu, S., Hou, X., Li, Z."Low risk HPV‑6E6 induces apoptosis in bone marrow‑derived dendritic cells". Oncology Letters 15, no. 1 (2018): 1157-1162. https://doi.org/10.3892/ol.2017.7417