Open Access

Knockdown of TBRG4 affects tumorigenesis in human H1299 lung cancer cells by regulating DDIT3, CAV1 and RRM2

  • Authors:
    • Ansheng Wang
    • Chengling Zhao
    • Xuegang Liu
    • Wen Su
    • Guixin Duan
    • Zongyu Xie
    • Shanshan Chu
    • Yuan Gao
  • View Affiliations

  • Published online on: November 2, 2017     https://doi.org/10.3892/ol.2017.7328
  • Pages:121-128
  • Copyright: © Wang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

Metrics: HTML 0 views | PDF 0 views
0

Abstract

The transforming growth factor β regulator 4 (TBRG4) gene, located on the 7p14‑p13 chromosomal region, is implicated in numerous types of cancer. However, the contribution(s) of TBRG4 in human lung cancer remains unknown. In the present study, the expression of TBRG4 mRNA was investigated in the H1299 lung cancer cell line using the quantitative polymerase chain reaction (qPCR) following the knockdown of TBRG4 by a lentivirus‑mediated small interfering RNA (siRNA). Results identified that the expression of TBRG4 within H1299 cells was significantly suppressed (P<0.01) by RNA interference, and 586 genes were differentially expressed following TBRG4 silencing. Ingenuity Pathway Analysis (IPA) revealed that these genes were often associated with infectious diseases, organismal injury, abnormalities and cancer functional networks. Further IPA of these networks revealed that TBRG4 knockdown in H1299 cells deregulated the expression of 21 downstream genes, including the upregulation of DNA damage‑inducible transcript 3 (DDIT3), also termed CCAAT/enhancer‑binding protein homologous protein, and downregulation of caveolin 1 (CAV1) and ribonucleotide reductase regulatory subunit M2 (RRM2). Results were validated using qPCR and western blotting. Furthermore, immunohistochemical staining of TBRG4 protein identified that expression was markedly increased in carcinoma compared with in normal tissue. In conclusion, TBRG4 serves a role in the tumorigenesis of lung cancer via deregulation of DDIT3, CAV1 and RRM2. The results of the present study may be important in contributing to our understanding of TBRG4 as a target for lung cancer treatment.

Related Articles

Journal Cover

January 2018
Volume 15 Issue 1

Print ISSN: 1792-1074
Online ISSN:1792-1082

2016 Impact Factor: 1.39
Ranked #68/217 Oncology
(total number of cites)

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
APA
Wang, A., Zhao, C., Liu, X., Su, W., Duan, G., Xie, Z. ... Gao, Y. (2018). Knockdown of TBRG4 affects tumorigenesis in human H1299 lung cancer cells by regulating DDIT3, CAV1 and RRM2. Oncology Letters, 15, 121-128. https://doi.org/10.3892/ol.2017.7328
MLA
Wang, A., Zhao, C., Liu, X., Su, W., Duan, G., Xie, Z., Chu, S., Gao, Y."Knockdown of TBRG4 affects tumorigenesis in human H1299 lung cancer cells by regulating DDIT3, CAV1 and RRM2". Oncology Letters 15.1 (2018): 121-128.
Chicago
Wang, A., Zhao, C., Liu, X., Su, W., Duan, G., Xie, Z., Chu, S., Gao, Y."Knockdown of TBRG4 affects tumorigenesis in human H1299 lung cancer cells by regulating DDIT3, CAV1 and RRM2". Oncology Letters 15, no. 1 (2018): 121-128. https://doi.org/10.3892/ol.2017.7328