Characteristics of doxorubicin-selected multidrug-resistant human leukemia HL‑60 cells with tolerance to arsenic trioxide and contribution of leukemia stem cells

  • Authors:
    • Jing Chen
    • Hulai Wei
    • Jie Cheng
    • Bei Xie
    • Bei Wang
    • Juan Yi
    • Baoying Tian
    • Zhuan Liu
    • Feifei Wang
    • Zhewen Zhang
  • View Affiliations

  • Published online on: November 6, 2017     https://doi.org/10.3892/ol.2017.7353
  • Pages:1255-1262
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Abstract

The present study selected and characterized a multidrug‑resistant HL‑60 human acute promyelocytic leukemia cell line, HL‑60/RS, by exposure to stepwise incremental doses of doxorubicin. The drug‑resistant HL‑60/RS cells exhibited 85.68‑fold resistance to doxorubicin and were cross‑resistant to other chemotherapeutics, including cisplatin, daunorubicin, cytarabine, vincristine and etoposide. The cells over‑expressed the transporters P‑glycoprotein, multidrug‑resistance‑related protein 1 and breast‑cancer‑resistance protein, encoded by the adenosine triphosphate‑binding cassette (ABC)B1, ABCC1 and ABCG2 genes, respectively. Unlike other recognized chemoresistant leukemia cell lines, HL‑60/RS cells were also strongly cross‑resistant to arsenic trioxide. The proportion of leukemia stem cells (LSCs) increased synchronously with increased of drug resistance in the doxorubicin‑induced HL‑60 cell population. The present study confirmed that doxorubicin‑induced HL‑60 cells exhibited multidrug‑resistance and high arsenic‑trioxide resistance. Drug‑resistance in these cells may be due to surviving chemoresistant LSCs in the HL‑60 population, which have been subjected to long and consecutive selection by doxorubicin.

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January 2018
Volume 15 Issue 1

Print ISSN: 1792-1074
Online ISSN:1792-1082

2016 Impact Factor: 1.39
Ranked #68/217 Oncology
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APA
Chen, J., Wei, H., Cheng, J., Xie, B., Wang, B., Yi, J. ... Zhang, Z. (2018). Characteristics of doxorubicin-selected multidrug-resistant human leukemia HL‑60 cells with tolerance to arsenic trioxide and contribution of leukemia stem cells. Oncology Letters, 15, 1255-1262. https://doi.org/10.3892/ol.2017.7353
MLA
Chen, J., Wei, H., Cheng, J., Xie, B., Wang, B., Yi, J., Tian, B., Liu, Z., Wang, F., Zhang, Z."Characteristics of doxorubicin-selected multidrug-resistant human leukemia HL‑60 cells with tolerance to arsenic trioxide and contribution of leukemia stem cells". Oncology Letters 15.1 (2018): 1255-1262.
Chicago
Chen, J., Wei, H., Cheng, J., Xie, B., Wang, B., Yi, J., Tian, B., Liu, Z., Wang, F., Zhang, Z."Characteristics of doxorubicin-selected multidrug-resistant human leukemia HL‑60 cells with tolerance to arsenic trioxide and contribution of leukemia stem cells". Oncology Letters 15, no. 1 (2018): 1255-1262. https://doi.org/10.3892/ol.2017.7353