Preliminary screening and identification of differentially expressed metastasis‑related ncRNAs in ovarian cancer

Yang,Yu; Zhang,Hui; Xie,Yajiao; Zhang,Shufen; Zhu,Junyou; Yin,Gang; Shu,Guang; Zhang,Yu

doi:10.3892/ol.2017.7338

Preliminary screening and identification of differentially expressed metastasis‑related ncRNAs in ovarian cancer

Authors:
- Yu Yang
- Hui Zhang
- Yajiao Xie
- Shufen Zhang
- Junyou Zhu
- Gang Yin
- Guang Shu
- Yu Zhang
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Affiliations: School of Resources Processing and Bioengineering, Central South University, Changsha, Hunan 410006, P.R. China, Department of Obstetrics and Gynecology, Xiangya Hospital, Central South University, Changsha, Hunan 410008, P.R. China, Xiangya Hospital, Central South University, Changsha, Hunan 410013, P.R. China
Published online on: November 2, 2017 https://doi.org/10.3892/ol.2017.7338
Pages: 368-374
Copyright: © Yang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Ovarian cancer (OC) is an aggressive disease with few valuable biomarkers and effective therapies. In this study, we aimed to elucidate biomarkers associated with OC metastasis into the omentum. We performed comprehensive screening of non‑coding RNAs (ncRNAs) between matched primary OC and omental metastasis using the Agilent human lncRNA Array V3.0 microarray. Reverse transcription quantitative polymerase chain reaction (RT‑qPCR) was used to validate the microarray results at the mRNA level. Microarray revealed 235 ncRNAs changes, and we validated the top four differential changed genes in an additional 27 paired samples with RT‑qPCR. We found that myocardial infarction associated transcript (MIAT) expression increased in the omentum tissue, while small nucleolar RNA, C/D Box 114 cluster (SNORD114) family members SNORD114‑10, SNORD114‑2 and SNORD114‑11 were downregulated when compared with OC tissue. However, there is no significant difference in SNORD114‑2 and SNORD114‑11 levels. We thus infer that differential expression of MIAT and SNORD114‑10 could play an important role during OC metastasis. These ncRNAs might be useful as pre‑diagnostic biomarkers at the early stage of cancer metastasis.

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