Open Access

Utility of bronchial lavage fluids for epithelial growth factor receptor mutation assay in lung cancer patients: Comparison between cell pellets, cell blocks and matching tissue specimens

  • Authors:
    • Shiho Asaka
    • Akihiko Yoshizawa
    • Rie Nakata
    • Tatsuya Negishi
    • Hiroshi Yamamoto
    • Takayuki Shiina
    • Shohei Shigeto
    • Kazuyuki Matsuda
    • Yukihiro Kobayashi
    • Takayuki Honda
  • View Affiliations

  • Published online on: November 21, 2017     https://doi.org/10.3892/ol.2017.7464
  • Pages:1469-1474
  • Copyright: © Asaka et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

The detection of epidermal growth factor receptor (EGFR) mutations is necessary for the selection of suitable patients with non‑small cell lung cancer (NSCLC) for treatment with EGFR tyrosine kinase inhibitors. Cytology specimens are known to be suitable for EGFR mutation detection, although tissue specimens should be prioritized; however, there are limited studies that examine the utility of bronchial lavage fluid (BLF) in mutation detection. The purpose of the present study was to investigate the utility of BLF specimens for the detection of EGFR mutations using a conventional quantitative EGFR polymerase chain reaction (PCR) assay. Initially, quantification cycle (Cq) values of cell pellets, cell‑free supernatants and cell blocks obtained from three series of 1% EGFR mutation‑positive lung cancer cell line samples were compared for mutation detection. In addition, PCR analysis of BLF specimens obtained from 77 consecutive NSCLC patients, detecting EGFR mutations was validated, and these results were compared with those for the corresponding formalin‑fixed paraffin‑embedded (FFPE) tissue specimens obtained by surgical resection or biopsy of 49 of these patients. The Cq values for mutation detection were significantly lower in the cell pellet group (average, 29.58) compared with the other groups, followed by those in cell‑free supernatants (average, 34.15) and in cell blocks (average, 37.12) for all three series (P<0.05). Mutational status was successfully analyzed in 77 BLF specimens, and the results obtained were concordant with those of the 49 matching FFPE tissue specimens. Notably, EGFR mutations were even detected in 10 cytological specimens that contained insufficient tumor cells. EGFR mutation testing with BLF specimens is therefore a useful and reliable method, particularly when sufficient cancer cells are not obtained.

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February 2018
Volume 15 Issue 2

Print ISSN: 1792-1074
Online ISSN:1792-1082

2016 Impact Factor: 1.39
Ranked #68/217 Oncology
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APA
Asaka, S., Yoshizawa, A., Nakata, R., Negishi, T., Yamamoto, H., Shiina, T. ... Honda, T. (2018). Utility of bronchial lavage fluids for epithelial growth factor receptor mutation assay in lung cancer patients: Comparison between cell pellets, cell blocks and matching tissue specimens. Oncology Letters, 15, 1469-1474. https://doi.org/10.3892/ol.2017.7464
MLA
Asaka, S., Yoshizawa, A., Nakata, R., Negishi, T., Yamamoto, H., Shiina, T., Shigeto, S., Matsuda, K., Kobayashi, Y., Honda, T."Utility of bronchial lavage fluids for epithelial growth factor receptor mutation assay in lung cancer patients: Comparison between cell pellets, cell blocks and matching tissue specimens". Oncology Letters 15.2 (2018): 1469-1474.
Chicago
Asaka, S., Yoshizawa, A., Nakata, R., Negishi, T., Yamamoto, H., Shiina, T., Shigeto, S., Matsuda, K., Kobayashi, Y., Honda, T."Utility of bronchial lavage fluids for epithelial growth factor receptor mutation assay in lung cancer patients: Comparison between cell pellets, cell blocks and matching tissue specimens". Oncology Letters 15, no. 2 (2018): 1469-1474. https://doi.org/10.3892/ol.2017.7464