Open Access

The association between differentially expressed micro RNAs in breast cancer cell lines and the micro RNA‑205 gene polymorphism in breast cancer tissue

  • Authors:
    • Jingcheng Zhang
    • Bin Wei
    • Huixian Hu
    • Fanrong Liu
    • Yan Tu
    • Fang He
  • View Affiliations

  • Published online on: December 8, 2017     https://doi.org/10.3892/ol.2017.7550
  • Pages:2139-2146
  • Copyright: © Zhang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

Metrics: HTML 0 views | PDF 0 views     Cited By: 0 citations

Abstract

Micro (mi)RNAs are an endogenous non‑coding small RNA comprised of 19‑26 nucleotides. miRNAs regulate gene expression through the recognition of its ‘seed sequence’ and interactions with 3'‑untranslated region of target miRNAs. Previous studies identified that miRNAs are associated with the onset and development of breast cancer and that a number of mutations in the coding DNA sequence of miRNAs affect its expression. Therefore, the present study aimed to screen differentially expressed miRNAs using miRNA expression profile chips to analyze the expression of miRNA (miR)‑205 in 12 breast cell lines of different metastatic performance and benign proliferative variation as well as breast cancer via in‑situ hybridization, and screen out single‑nucleotide polymorphisms (SNPs) in the miR‑205 coding gene region. In addition, the association between SNP and the clinicopathological features of breast lesions was investigated. The results of the present study demonstrated that the expression of miR‑205 was decreased in breast cancer cells and tissues compared with benign lesions. SNPs in the miR‑205 coding gene region were identified in tumor cell lines and as increasing lengths of the AGC repetitive sequence were lost, miR‑205 expression increased. An association was identified between a number of SNPs in the miR‑205 coding gene region and breast cancer, as well as between SNPs in miR‑205 coding gene region and the clinicopathological features of breast cancer.

Related Articles

Journal Cover

February 2018
Volume 15 Issue 2

Print ISSN: 1792-1074
Online ISSN:1792-1082

2016 Impact Factor: 1.39
Ranked #68/217 Oncology
(total number of cites)

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
APA
Zhang, J., Wei, B., Hu, H., Liu, F., Tu, Y., & He, F. (2018). The association between differentially expressed micro RNAs in breast cancer cell lines and the micro RNA‑205 gene polymorphism in breast cancer tissue. Oncology Letters, 15, 2139-2146. https://doi.org/10.3892/ol.2017.7550
MLA
Zhang, J., Wei, B., Hu, H., Liu, F., Tu, Y., He, F."The association between differentially expressed micro RNAs in breast cancer cell lines and the micro RNA‑205 gene polymorphism in breast cancer tissue". Oncology Letters 15.2 (2018): 2139-2146.
Chicago
Zhang, J., Wei, B., Hu, H., Liu, F., Tu, Y., He, F."The association between differentially expressed micro RNAs in breast cancer cell lines and the micro RNA‑205 gene polymorphism in breast cancer tissue". Oncology Letters 15, no. 2 (2018): 2139-2146. https://doi.org/10.3892/ol.2017.7550