Activin A induces apoptosis of mouse myeloma cells via the mitochondrial pathway

  • Authors:
    • Yuanyi Zhang
    • Yan Qi
    • Yang Zhao
    • Hongyan Sun
    • Jingyan Ge
    • Zhonghui Liu
  • View Affiliations

  • Published online on: December 11, 2017     https://doi.org/10.3892/ol.2017.7584
  • Pages:2590-2594
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Abstract

Activin A is a pleiotropic cytokine belonging to the transforming growth factor β superfamily. Abnormal expression of activin A is associated with tumorigenesis. Multiple myeloma is characterized by the development of osteolytic disease, which ultimately leads to cachexia. However, the involvement of activin A in myeloma cell viability and apoptosis remains to be fully elucidated. For this purpose, mouse myeloma NS‑1 cells were treated with activin A, and subsequently subjected to 5‑bromo‑2'‑deoxyuridine analysis, Hoechst 33342 staining, flow cytometry and western blot analysis. The results revealed that activin A significantly suppressed NS‑1 cell viability, and induced NS‑1 cell apoptosis. In addition, activin A‑induced promotion of NS‑1 cell apoptosis was accompanied by upregulated expression of BCL2 associated X, apoptosis regulator (Bax), but downregulated expression of B cell lymphoma‑2 (Bcl‑2), resulting in an increase of the Bax/Bcl‑2 ratio. Furthermore, cytochrome c and caspase‑3 protein expression also increased following treatment with activin A. These data suggest that activin A induces apoptosis in mouse myeloma NS‑1 cells via the mitochondrial pathway, providing a novel insight into multiple myeloma treatment.

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February 2018
Volume 15 Issue 2

Print ISSN: 1792-1074
Online ISSN:1792-1082

2016 Impact Factor: 1.39
Ranked #68/217 Oncology
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APA
Zhang, Y., Qi, Y., Zhao, Y., Sun, H., Ge, J., & Liu, Z. (2018). Activin A induces apoptosis of mouse myeloma cells via the mitochondrial pathway. Oncology Letters, 15, 2590-2594. https://doi.org/10.3892/ol.2017.7584
MLA
Zhang, Y., Qi, Y., Zhao, Y., Sun, H., Ge, J., Liu, Z."Activin A induces apoptosis of mouse myeloma cells via the mitochondrial pathway". Oncology Letters 15.2 (2018): 2590-2594.
Chicago
Zhang, Y., Qi, Y., Zhao, Y., Sun, H., Ge, J., Liu, Z."Activin A induces apoptosis of mouse myeloma cells via the mitochondrial pathway". Oncology Letters 15, no. 2 (2018): 2590-2594. https://doi.org/10.3892/ol.2017.7584