Open Access

MicroRNA‑9 functions as a tumor suppressor and enhances radio‑sensitivity in radio‑resistant A549 cells by targeting neuropilin 1

  • Authors:
    • Kai Xiong
    • Li Hong Shao
    • Hai Qin Zhang
    • Linlin Jin
    • Wei Wei
    • Zhuo Dong
    • Yue Quan Zhu
    • Ning Wu
    • Shun Zi Jin
    • Li Xiang Xue
  • View Affiliations

  • Published online on: December 28, 2017     https://doi.org/10.3892/ol.2017.7705
  • Pages: 2863-2870
  • Copyright: © Xiong et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Radiotherapy is commonly used to treat lung cancer but may not kill all cancer cells, which may be attributed to the radiotherapy resistance that often occurs in non‑small cell lung cancer (NSCLC). At present, the molecular mechanism of radio‑resistance remains unclear. Neuropilin 1 (NRP1), a co‑receptor for vascular endothelial growth factor (VEGF), was demonstrated to be associated with radio‑resistance of NSCLC cells via the VEGF‑phosphoinositide 3‑kinase‑nuclear factor‑κB pathway in our previous study. It was hypothesized that certain microRNAs (miRs) may serve crucial functions in radio‑sensitivity by regulating NRP1. Bioinformatics predicted that NRP1 was a potential target of miR‑9, and this was validated by luciferase reporter assays. Functionally, miR‑9‑transfected A549 cells exhibited a decreased proliferation rate, increased apoptosis rate and attenuated migratory and invasive abilities. Additionally, a high expression of miR‑9 also significantly enhanced the radio‑sensitivity of A549 cells in vitro and in vivo. These data improve understanding of the mechanisms of cell radio‑resistance, and suggest that miR‑9 may be a molecular target for the prediction of radio‑sensitivity in NSCLC.
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March-2018
Volume 15 Issue 3

Print ISSN: 1792-1074
Online ISSN:1792-1082

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Copy and paste a formatted citation
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Spandidos Publications style
Xiong K, Shao L, Zhang H, Jin L, Wei W, Dong Z, Zhu Y, Wu N, Jin S, Xue L, Xue L, et al: MicroRNA‑9 functions as a tumor suppressor and enhances radio‑sensitivity in radio‑resistant A549 cells by targeting neuropilin 1. Oncol Lett 15: 2863-2870, 2018
APA
Xiong, K., Shao, L., Zhang, H., Jin, L., Wei, W., Dong, Z. ... Xue, L. (2018). MicroRNA‑9 functions as a tumor suppressor and enhances radio‑sensitivity in radio‑resistant A549 cells by targeting neuropilin 1. Oncology Letters, 15, 2863-2870. https://doi.org/10.3892/ol.2017.7705
MLA
Xiong, K., Shao, L., Zhang, H., Jin, L., Wei, W., Dong, Z., Zhu, Y., Wu, N., Jin, S., Xue, L."MicroRNA‑9 functions as a tumor suppressor and enhances radio‑sensitivity in radio‑resistant A549 cells by targeting neuropilin 1". Oncology Letters 15.3 (2018): 2863-2870.
Chicago
Xiong, K., Shao, L., Zhang, H., Jin, L., Wei, W., Dong, Z., Zhu, Y., Wu, N., Jin, S., Xue, L."MicroRNA‑9 functions as a tumor suppressor and enhances radio‑sensitivity in radio‑resistant A549 cells by targeting neuropilin 1". Oncology Letters 15, no. 3 (2018): 2863-2870. https://doi.org/10.3892/ol.2017.7705