Open Access

Effect of galectin-3 on vasculogenic mimicry in esophageal cancer cells

Retraction in: /10.3892/ol.2018.9643

  • Authors:
    • Jingxin Zhang
    • Guodong Deng
    • Lili Qiao
    • Hui Luo
    • Qiqi Liu
    • Ning Liang
    • Jian Xie
    • Jiandong Zhang
  • View Affiliations

  • Published online on: February 6, 2018     https://doi.org/10.3892/ol.2018.7959
  • Pages: 4907-4911
  • Copyright: © Zhang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

Metrics: HTML 0 views | PDF 0 views     Cited By (CrossRef): 0 citations

Abstract

Galectin-3 is a multifunctional β-galactoside binding lectin associated with tumor progression. Previous studies confirmed the roles of galecin‑3 overexpression and silencing in the biological behavior of Eca109 human esophageal cancer (EC) cells; galectin‑3 may serve a critical role in the vasculogenic mimicry (VM) of tumors. Therefore, the present study examined the effects of galectin‑3 knockdown using lentivirus vectors on VM in EC. Eca109 and EC9706 EC cells were transfected with a lentiviral vector to inhibit galectin‑3 expression, or a control vector. VM formation in vitro was evaluated via 3D culture. Western blotting was used to detect the expression level of galectin‑3 following galectin‑3 silencing and the expression levels of VE‑cadherin, ephrin type‑A receptor 2 precursor (EphA2) and matrix metalloproteinase 2 (MMP‑2). According to the results of western blot analysis, the Eca109/galectin‑3 and EC9706/galectin‑3 cells exhibited effective galectin‑3 silencing (P<0.05). Eca109 and EC9706 cells formed typical tubular networks; the number of tubular networks markedly decreased subsequent to galectin‑3 knockdown. The expression levels of MMP‑2 and EphA2 proteins in Eca109/galectin‑3 and EC9706/galectin‑3 cells were lower compared with those in Eca109, EC9706, and control vector‑transfected Eca109 and EC9706 cells (P<0.05); however, there was no significant difference in the expression of VE‑cadherin proteins. These results indicated that galectin‑3 may modulate VM in EC by regulating the EphA2 expression level, which affects VM formation via MMP-2.

Related Articles

Journal Cover

April 2018
Volume 15 Issue 4

Print ISSN: 1792-1074
Online ISSN:1792-1082

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
APA
Zhang, J., Deng, G., Qiao, L., Luo, H., Liu, Q., Liang, N. ... Zhang, J. (2018). Effect of galectin-3 on vasculogenic mimicry in esophageal cancer cells Retraction in /10.3892/ol.2018.9643. Oncology Letters, 15, 4907-4911. https://doi.org/10.3892/ol.2018.7959
MLA
Zhang, J., Deng, G., Qiao, L., Luo, H., Liu, Q., Liang, N., Xie, J., Zhang, J."Effect of galectin-3 on vasculogenic mimicry in esophageal cancer cells Retraction in /10.3892/ol.2018.9643". Oncology Letters 15.4 (2018): 4907-4911.
Chicago
Zhang, J., Deng, G., Qiao, L., Luo, H., Liu, Q., Liang, N., Xie, J., Zhang, J."Effect of galectin-3 on vasculogenic mimicry in esophageal cancer cells Retraction in /10.3892/ol.2018.9643". Oncology Letters 15, no. 4 (2018): 4907-4911. https://doi.org/10.3892/ol.2018.7959