MicroRNA‑124 acts as a tumor‑suppressive miRNA by inhibiting the expression of Snail2 in osteosarcoma
- Jianghong Huang
- Yujie Liang
- Meiquan Xu
- Jianyi Xiong
- Daping Wang
- Qiang Ding
Published online on: February 8, 2018
Copyright: © Huang et al.
This is an open access article distributed under the terms of Creative Commons Attribution License.
The aim of the present study was to investigate the clinical significance of hsa-microRNA-124-3p (miR-124) in osteosarcoma (OS), and examine its role in cell growth and invasion. Using a microRNA chip array, the expression of miR‑124 was detected in samples of surgically resected OS and matched against the levels of expression in tumor‑adjacent normal tissues. The levels of miR‑124 were upregulated in the OS cells through the transfection of miR‑124 mimics. Cell proliferation and Transwell assays were performed to determine cell proliferation and invasion; Reverse transcription-quantitative polymerase chain reaction, western blot and luciferase assays were then used to detect the expression of the target gene snail family zinc finger 2 (Snail2). The expression of miR‑124 was significantly lower in the OS tissues, compared with that in the tumor‑adjacent normal tissues; and the expression of miR‑124 in the tumor tissues was significantly associated with tumor size. miR‑124 directly repressed the expression of Snail2, and resulted in a significant inhibition of cell proliferation and invasion. In a mouse model, the overexpression of miR‑124 significantly inhibited U2OS cell proliferation and invasion. Taken together, miR‑124 was associated with the adverse clinical and pathological features observed in OS. It acted as a tumor suppressor to regulate the proliferation and invasion of OS cells by targeting Snail2, suggesting that miR‑124 may be key in the progression of OS.