Pro-apoptotic effect of TRAIL-transfected endothelial progenitor cells on glioma cells
- Xin Deng
- Wen Zhao
- Laijun Song
- Wei Ying
- Xinbin Guo
Published online on: February 7, 2018
Copyright: © Deng et al.
This is an open access article distributed under the terms of Creative Commons Attribution License.
Glioma is one of the most common aggressive neuroepithelial malignant tumors in the central nervous system. It has a high recurrence rate and poor prognosis, primarily due to the fact that novel therapeutic agents cannot penetrate the blood‑brain barrier (BBB). Endothelial progenitor cells (EPCs) have been reported to move across the BBB and access the tumor site. However, whether EPCs expressing the tumor necrosis factor‑related apoptosis‑inducing ligand (TRAIL) induce glioma cell apoptosis requires further investigation. In the present study, EPCs were transfected and stably expressed with TRAIL through lentiviral infection. The pro‑apoptotic effect of these TRAIL‑expressing EPCs on the SHG44 glioma cell line was investigated. The migration ability of TRAIL‑expressing EPCs toward SHG44 cells through the Transwell culture system was investigated via a high‑content screening assay. The apoptotic rate and the expression of cleaved caspase‑8 and ‑3 in addition to the cleaved poly(ADP‑ribose) polymerase in SHG44 cells significantly increased in the TRAIL‑overexpressing EPC treatment group compared with the controls. The increased apoptotic rate was reversed using a caspase inhibitor. The findings suggested that the TRAIL‑expressing EPCs induced apoptosis in the SHG44 cells by activating the death receptor pathway, indicating that the TRAIL‑expressing EPCs may be a useful strategy for glioma treatment.