Post-operative unadjuvanted therapeutic xenovaccination with chicken whole embryo vaccine suppresses distant micrometastases and prolongs survival in a murine Lewis lung carcinoma model

  • Authors:
    • Jan Aleksander Kraśko
    • Karolina Žilionytė
    • Adas Darinskas
    • Neringa Dobrovolskienė
    • Agata Mlynska
    • Svetlana Riabceva
    • Iosif Zalutsky
    • Marina Derevyanko
    • Vladimir Kulchitsky
    • Olga Karaman
    • Natalia Fedosova
    • Tatiana Vasyliyvna Symchych
    • Gennady Didenko
    • Vasyl Chekhun
    • Marius Strioga
    • Vita Pašukonienė
  • View Affiliations

  • Published online on: February 5, 2018     https://doi.org/10.3892/ol.2018.7950
  • Pages: 5098-5104
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Abstract

Immunotherapy in the form of anticancer vaccination relies on the mobilization of the patient's immune system against specific cancer antigens. Instead of focusing on an autologous cell lysate, which is not always available in clinical practice, the present study investigates vaccines utilizing xenogeneic foetal tissue that are rich in oncofoetal antigens. Lewis lung carcinoma (LLC)‑challenged C57BL/6 mice were treated with either a xenogeneic vaccine made from chicken whole embryo, or a xenogeneic vaccine made from rat embryonic brain tissue, supplemented with a Bacillus subtilis protein fraction as an adjuvant. Median and overall survival, size of metastatic foci in lung tissue and levels of circulating CD8a+ T cells were evaluated and compared with untreated control mice. Following primary tumour removal, a course of three subcutaneous vaccinations with xenogeneic chicken embryo vaccine led to significant increase in overall survival rate (100% after 70 days of follow‑up vs. 40% in untreated control mice), significant increase in circulating CD8a+ T cells (18.18 vs. 12.6% in untreated control mice), and a significant decrease in the area and incidence of metastasis foci. The xenogeneic rat brain tissue‑based vaccine did not improve any of the investigated parameters, despite promising reports in other models. We hypothesize that the proper selection of antigen source (tissue) can constitute an effective immunotherapeutic product.

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April 2018
Volume 15 Issue 4

Print ISSN: 1792-1074
Online ISSN:1792-1082

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APA
Kraśko, J.A., Žilionytė, K., Darinskas, A., Dobrovolskienė, N., Mlynska, A., Riabceva, S. ... Pašukonienė, V. (2018). Post-operative unadjuvanted therapeutic xenovaccination with chicken whole embryo vaccine suppresses distant micrometastases and prolongs survival in a murine Lewis lung carcinoma model. Oncology Letters, 15, 5098-5104. https://doi.org/10.3892/ol.2018.7950
MLA
Kraśko, J. A., Žilionytė, K., Darinskas, A., Dobrovolskienė, N., Mlynska, A., Riabceva, S., Zalutsky, I., Derevyanko, M., Kulchitsky, V., Karaman, O., Fedosova, N., Symchych, T. V., Didenko, G., Chekhun, V., Strioga, M., Pašukonienė, V."Post-operative unadjuvanted therapeutic xenovaccination with chicken whole embryo vaccine suppresses distant micrometastases and prolongs survival in a murine Lewis lung carcinoma model". Oncology Letters 15.4 (2018): 5098-5104.
Chicago
Kraśko, J. A., Žilionytė, K., Darinskas, A., Dobrovolskienė, N., Mlynska, A., Riabceva, S., Zalutsky, I., Derevyanko, M., Kulchitsky, V., Karaman, O., Fedosova, N., Symchych, T. V., Didenko, G., Chekhun, V., Strioga, M., Pašukonienė, V."Post-operative unadjuvanted therapeutic xenovaccination with chicken whole embryo vaccine suppresses distant micrometastases and prolongs survival in a murine Lewis lung carcinoma model". Oncology Letters 15, no. 4 (2018): 5098-5104. https://doi.org/10.3892/ol.2018.7950