Novel TLR7 agonist stimulates activity of CIK/NK immunological effector cells to enhance antitumor cytotoxicity

  • Authors:
    • Dong Gao
    • Yongguang Cai
    • Yanyuan Chen
    • Wang Li
    • Chih‑Chang Wei
    • Xiaoling Luo
    • Yuhuan Wang
  • View Affiliations

  • Published online on: February 5, 2018     https://doi.org/10.3892/ol.2018.7954
  • Pages: 5105-5110
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Abstract

Toll-like receptor (TLR) 7/8 agonists have been applied in combination with chemo‑, radio‑ or immunotherapy for lymphoma, and used as topical drugs for the treatment of viral skin lesions and skin tumors. In the present study, the role of an adenine analog, 9‑(4‑carboxyphenyl)‑8‑hydroxy‑2‑(2‑methoxyethoxy)‑adenine [termed Gao Dong (GD)], a novel TLR7 agonist, in the activation of cytokine‑induced killer/natural killer (CIK/NK) cells was determined. The results of the present study indicated that GD was able to activate CIK/NK cells. The proportion of GD‑induced CD3+CD56+ CIK and CD3‑CD56+ NK cells was ~4% higher respectively compared with the control. Notably, combination therapy with CIK/NK cells stimulated by GD, markedly suppressed the proliferation of the chronic myelogenous leukemia K562 cell line. Following GD treatment, the cytotoxicity improved by ~25 and 21% when the effector/target ratio was 20:1 and 10:1, respectively. The results of the present study suggested a novel protocol for CIK/NK cell proliferation and revealed that GD may serve as a potent innate and adaptive immunomodulator in immunocyte culture.
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April-2018
Volume 15 Issue 4

Print ISSN: 1792-1074
Online ISSN:1792-1082

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Spandidos Publications style
Gao D, Cai Y, Chen Y, Li W, Wei CC, Luo X and Wang Y: Novel TLR7 agonist stimulates activity of CIK/NK immunological effector cells to enhance antitumor cytotoxicity. Oncol Lett 15: 5105-5110, 2018
APA
Gao, D., Cai, Y., Chen, Y., Li, W., Wei, C., Luo, X., & Wang, Y. (2018). Novel TLR7 agonist stimulates activity of CIK/NK immunological effector cells to enhance antitumor cytotoxicity. Oncology Letters, 15, 5105-5110. https://doi.org/10.3892/ol.2018.7954
MLA
Gao, D., Cai, Y., Chen, Y., Li, W., Wei, C., Luo, X., Wang, Y."Novel TLR7 agonist stimulates activity of CIK/NK immunological effector cells to enhance antitumor cytotoxicity". Oncology Letters 15.4 (2018): 5105-5110.
Chicago
Gao, D., Cai, Y., Chen, Y., Li, W., Wei, C., Luo, X., Wang, Y."Novel TLR7 agonist stimulates activity of CIK/NK immunological effector cells to enhance antitumor cytotoxicity". Oncology Letters 15, no. 4 (2018): 5105-5110. https://doi.org/10.3892/ol.2018.7954