A LY-15, a novel cyclic pentapeptide that inhibits B16 cell proliferation and migration and induces cell apoptosis

Wang,Xuan; Yao,Xiangli; Fan,Shiming; Xiang,Chenshuang; Liu,Runjiao; Feng,Juan; Huang,Jing; Liu,Shouxin

doi:10.3892/ol.2018.8023

A LY-15, a novel cyclic pentapeptide that inhibits B16 cell proliferation and migration and induces cell apoptosis

Authors:
- Xuan Wang
- Xiangli Yao
- Shiming Fan
- Chenshuang Xiang
- Runjiao Liu
- Juan Feng
- Jing Huang
- Shouxin Liu
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Affiliations: State Key Laboratory Breeding Base‑Hebei Province Key Laboratory of Molecular Chemistry for Drug, Hebei University of Science and Technology, Shijiazhuang, Hebei 050018, P.R. China
Published online on: February 12, 2018 https://doi.org/10.3892/ol.2018.8023
Pages: 5887-5892

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Abstract

Melanoma is highly resistant to most traditional treatments; therefore, its incidence and mortality rates are rapidly increasing. The effect of a novel sansalvamide A analogue named LY-15 on the growth and induction of apoptosis in B16 cancer cells was investigated in vitro. The inhibitory effects of LY‑15 on B16 cells occurred in a concentration‑ and time‑dependent manner. The B16 cells were cultured in various concentrations of LY‑15 (5, 15 and 25 µM), and the ameliorating effect of LY‑15 was evaluated using apoptotic protein markers B‑cell lymphoma 2 (Bcl‑2), Bcl‑2‑associated X protein (Bax), caspase‑3 and caspase‑9. Furthermore, LY‑15 effectively inhibited the B16 cell migration, increased the expressions levels of caspase‑3, caspase‑9 and the pro‑apoptotic Bax, and reduced that of the anti‑apoptotic Bcl‑2. These findings suggested that LY‑15 is a promising chemotherapeutic agent against melanoma by inducing apoptosis through the mitochondrial‑associated death pathway. In addition, sansalvamide A analogue LY‑15 may a significant therapeutic target for the treatment of malignant melanoma cancer.

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