Open Access

Differential regulation of AKT1 contributes to survival and proliferation in hepatocellular carcinoma cells by mediating Notch1 expression

  • Authors:
    • Jing Chen
    • Jun Liang
    • Shihai Liu
    • Shanai Song
    • Wenxuan Guo
    • Fangzhen Shen
  • View Affiliations

  • Published online on: March 7, 2018     https://doi.org/10.3892/ol.2018.8193
  • Pages: 6857-6864
  • Copyright: © Chen et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

The RAC serine/threonine-protein kinase (AKT) family of serine/threonine protein kinases, particularly the AKT1 isoform, has been identified abnormally expressed in hepatocellular carcinoma (HCC) cells, and is highly associated with cell behavior, including proliferation, survival, metabolism, and tumorigenesis. However, the specific mechanism by which AKT1 elicits these effects requires further study. The purpose of the present study was to reveal the effects of AKT1 on the survival and proliferation of HCC cells, and to investigate the mechanisms involved. Western blotting and reverse transcription‑quantitative polymerase chain reaction (RT‑qPCR) to evaluate the expression levels of AKT1 in HCC SMMC‑7721 cell line. Molecular mechanisms and the influences of different regulation the expression of AKT1 on HCC cell growth, proliferation were determined by western blotting, MTT and colony formation assays, cell cycle and apoptosis were investigated by flow cytometry. The activation of AKT1 suppressed the expression of phosphatase and tensin homolog and increased the activation of Notch1. The inhibition of AKT1 effectively suppressed the expression of Notch1. Furthermore, the data of the present study indicated that B‑cell lymphoma 2 and cyclin D1 is involved in the regulation of AKT1 expression.
View Figures
View References

Related Articles

Journal Cover

May-2018
Volume 15 Issue 5

Print ISSN: 1792-1074
Online ISSN:1792-1082

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
x
Spandidos Publications style
Chen J, Liang J, Liu S, Song S, Guo W and Shen F: Differential regulation of AKT1 contributes to survival and proliferation in hepatocellular carcinoma cells by mediating Notch1 expression. Oncol Lett 15: 6857-6864, 2018
APA
Chen, J., Liang, J., Liu, S., Song, S., Guo, W., & Shen, F. (2018). Differential regulation of AKT1 contributes to survival and proliferation in hepatocellular carcinoma cells by mediating Notch1 expression. Oncology Letters, 15, 6857-6864. https://doi.org/10.3892/ol.2018.8193
MLA
Chen, J., Liang, J., Liu, S., Song, S., Guo, W., Shen, F."Differential regulation of AKT1 contributes to survival and proliferation in hepatocellular carcinoma cells by mediating Notch1 expression". Oncology Letters 15.5 (2018): 6857-6864.
Chicago
Chen, J., Liang, J., Liu, S., Song, S., Guo, W., Shen, F."Differential regulation of AKT1 contributes to survival and proliferation in hepatocellular carcinoma cells by mediating Notch1 expression". Oncology Letters 15, no. 5 (2018): 6857-6864. https://doi.org/10.3892/ol.2018.8193