Open Access

Comparison of the algorithms classifying the ABC and GCB subtypes in diffuse large B-cell lymphoma

  • Authors:
    • Lučka Boltežar
    • Veronika Kloboves Prevodnik
    • Maja Pohar Perme
    • Gorana Gašljević
    • Barbara Jezeršek Novaković
  • View Affiliations

  • Published online on: March 12, 2018     https://doi.org/10.3892/ol.2018.8243
  • Pages: 6903-6912
  • Copyright: © Boltežar et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Different immunohistochemical algorithms for the classification of the activated B‑cell (ABC) and germinal center B‑cell (GCB) subtypes of diffuse large B‑cell lymphoma (DLBCL) are applied in different laboratories. In the present study, 127 patients with DLCBL were investigated, all treated with rituximab and cyclophosphamide, hydroxydaunorubicin, oncovin and prednisone (CHOP) or CHOP‑like regimens between April 2004 and December 2010. Multi‑tumor tissue microarrays were prepared and were tested according to 4 algorithms: Hans; modified Hans; Choi; and modified Choi. For 39 patients, the flow cytometric quantification of CD19 and CD20 antigen expression was performed and the level of expression presented as molecules of equivalent soluble fluorochrome units. The Choi algorithm was demonstrated to be prognostic for OS and classified patients into the GCB subgroup with an HR of 0.91. No difference in the expression of the CD19 antigen between the ABC and GCB groups was observed, but the ABC subtype exhibited a decreased expression of the CD20 antigen compared with the GCB subtype.
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May-2018
Volume 15 Issue 5

Print ISSN: 1792-1074
Online ISSN:1792-1082

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Spandidos Publications style
Boltežar L, Prevodnik VK, Perme MP, Gašljević G and Novaković BJ: Comparison of the algorithms classifying the ABC and GCB subtypes in diffuse large B-cell lymphoma. Oncol Lett 15: 6903-6912, 2018
APA
Boltežar, L., Prevodnik, V.K., Perme, M.P., Gašljević, G., & Novaković, B.J. (2018). Comparison of the algorithms classifying the ABC and GCB subtypes in diffuse large B-cell lymphoma. Oncology Letters, 15, 6903-6912. https://doi.org/10.3892/ol.2018.8243
MLA
Boltežar, L., Prevodnik, V. K., Perme, M. P., Gašljević, G., Novaković, B. J."Comparison of the algorithms classifying the ABC and GCB subtypes in diffuse large B-cell lymphoma". Oncology Letters 15.5 (2018): 6903-6912.
Chicago
Boltežar, L., Prevodnik, V. K., Perme, M. P., Gašljević, G., Novaković, B. J."Comparison of the algorithms classifying the ABC and GCB subtypes in diffuse large B-cell lymphoma". Oncology Letters 15, no. 5 (2018): 6903-6912. https://doi.org/10.3892/ol.2018.8243