miR-146a promotes cell migration and invasion in melanoma by directly targeting SMAD4

Pu,Wei; Shang,Yongming; Shao,Qiang; Yuan,Xinpeng

doi:10.3892/ol.2018.8172

miR-146a promotes cell migration and invasion in melanoma by directly targeting SMAD4

Authors:
- Wei Pu
- Yongming Shang
- Qiang Shao
- Xinpeng Yuan
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Affiliations: Department of Dermatology, The Central Hospital of Zibo, Zibo, Shandong 255000, P.R. China, Department of Dermatology, Zibo Traditional Chinese Medicine Hospital, Zibo, Shandong 255300, P.R. China
Published online on: March 5, 2018 https://doi.org/10.3892/ol.2018.8172
Pages: 7111-7117
Copyright: © Pu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Previous studies have explored the functions of microRNA (miR)-146a in different types of cancer through mediating different targets. However, the roles of miR‑146a in malignant melanoma (MM) cell migration and invasion remain largely elusive. In the present study, the potential molecular function of miR‑146a in MM was investigated. Reverse transcription‑quantitative polymerase chain reaction was utilized to detect miR‑146a expression in MM tissues and cell lines. A Transwell assay was performed to confirm the ability of migration and invasion. A luciferase assay and biological analysis were used to predict and determine the targets of miR‑146a. The expression of miR‑146a was upregulated in melanoma tissues and cell lines. Clinicopathological analysis indicated that the miR‑146a level was negatively correlated with the progression of melanoma. Abnormal expression of miR‑146a promoted cell migration and invasion in MM cells. Additionally, it was also observed that Mothers against decapentaplegic homolog 4 (SMAD4) was a novel target of miR‑146a in MM. SMAD4 was negatively associated with miR‑146a in MM and abnormal expression of SMAD4 attenuated miR‑146a‑mediated promotion of cell migration and invasion. In conclusion, miR‑146a functioned as an oncogene by directly targeting SMAD4 and it may be a novel diagnostic and therapeutic marker of MM.

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