Open Access

Overexpressing modified human TRβ1 suppresses the proliferation of breast cancer MDA‑MB‑468 cells

  • Authors:
    • Xiaoxiang Peng
    • Yangyang Zhang
    • Yanli Sun
    • Lujuan Wang
    • Wei Song
    • Qian Li
    • Ronglan Zhao
  • View Affiliations

  • Published online on: May 22, 2018     https://doi.org/10.3892/ol.2018.8764
  • Pages: 785-792
  • Copyright: © Peng et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

A number of studies have indicated that thyroid hormone receptor β1 (TRβ1) functions as a tumor suppressor. TRs mediate transcriptional responses through a highly conserved DNA‑binding domain (DBD). A novel rat TRβ isoform (rTRβΔ) was previously identified, in which a novel exon, N (108 bp), is located between exons 3 and 4 within the DBD; this exon represents the only difference between rTRβΔ and rTRβ1. In vitro, rTRβΔ exhibits a stronger tumor‑suppressive capacity than rTRβ1, and further analysis revealed a high level of conservation between the rat and human DBD sequences. In the present study, an artificially modified human TRβ1 (m‑hTRβ1) was constructed via the introduction of the 108‑bp sequence into the corresponding position of the wild‑type human TRβ1 (wt‑hTRβ1) DBD. An electrophoretic mobility shift assay and transfection experiments confirmed that m‑hTRβ1 is functional. Overexpression of m‑hTRβ1 inhibits the proliferation of MDA‑MB‑468 cells in the presence of triiodothyronine by promoting apoptosis, which may be associated with the upregulation of Caspase‑3 and Bak gene expression and the activation of the Caspase‑3 protein. In addition, the pro‑apoptotic effect of m‑hTRβ1 was stronger, compared with wt‑hTRβ1. These results indicated that m‑hTRβ1 may act as a tumor suppressor in MDA‑MB‑468 cells. These data provided a novel insight into gene therapy for breast cancer.
View Figures
View References

Related Articles

Journal Cover

July-2018
Volume 16 Issue 1

Print ISSN: 1792-1074
Online ISSN:1792-1082

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
x
Spandidos Publications style
Peng X, Zhang Y, Sun Y, Wang L, Song W, Li Q and Zhao R: Overexpressing modified human TRβ1 suppresses the proliferation of breast cancer MDA‑MB‑468 cells. Oncol Lett 16: 785-792, 2018
APA
Peng, X., Zhang, Y., Sun, Y., Wang, L., Song, W., Li, Q., & Zhao, R. (2018). Overexpressing modified human TRβ1 suppresses the proliferation of breast cancer MDA‑MB‑468 cells. Oncology Letters, 16, 785-792. https://doi.org/10.3892/ol.2018.8764
MLA
Peng, X., Zhang, Y., Sun, Y., Wang, L., Song, W., Li, Q., Zhao, R."Overexpressing modified human TRβ1 suppresses the proliferation of breast cancer MDA‑MB‑468 cells". Oncology Letters 16.1 (2018): 785-792.
Chicago
Peng, X., Zhang, Y., Sun, Y., Wang, L., Song, W., Li, Q., Zhao, R."Overexpressing modified human TRβ1 suppresses the proliferation of breast cancer MDA‑MB‑468 cells". Oncology Letters 16, no. 1 (2018): 785-792. https://doi.org/10.3892/ol.2018.8764