miR‑184 promotes cell proliferation in tongue squamous cell carcinoma by targeting SOX7

Chen,Daiyun; Li,Junfu; Li,Shunrong; Han,Ping; Li,Ning; Wang,Yi; Du,Shouqin

doi:10.3892/ol.2018.8906

miR‑184 promotes cell proliferation in tongue squamous cell carcinoma by targeting SOX7

Authors:
- Daiyun Chen
- Junfu Li
- Shunrong Li
- Ping Han
- Ning Li
- Yi Wang
- Shouqin Du
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Affiliations: Department of Stomatology, Affiliated Hospital of Taishan Medical University, Taian, Shandong 271000, P.R. China, Department of Critical Care Medicine, People's Hospital of Zhangqiu, Zhangqiu, Shandong 250200, P.R. China, Department of Respiratory Medicine, People's Hospital of Zhangqiu, Zhangqiu, Shandong 250200, P.R. China, Department of Pathology, People's Hospital of Zhangqiu, Zhangqiu, Shandong 250200, P.R. China
Published online on: June 5, 2018 https://doi.org/10.3892/ol.2018.8906
Pages: 2221-2228

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Abstract

The aim of this study was to investigate whether the miR‑184 could regulate the proliferation of the tongue squamous cell carcinoma (TSCC) through sex‑determining region Y‑box 7 (SOX7) gene. miR‑184 expression was upregulated in TSCC cell lines and tissues. MTT assay revealed that overexpression of miR‑184 significantly promoted the proliferation of the TSCC cells in vitro. SOX7 was the direct target of miR‑184 and luciferase reporter assay confirmed that miR‑184 downregulated the expression of SOX7. MTT assay verified that knockdown of SOX7 remarkably promoted the proliferation of TSCC cells in vitro. miR‑184 promoted the proliferation of TSCC by targeting SOX7. Taken together, our results provided a new potential therapeutic target for TSCC treatment.

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1	Wang W, Liu Z, Zhao L, Sun J, He Q, Yan W, Lu Z and Wang A: Hexokinase 2 enhances the metastatic potential of tongue squamous cell carcinoma via the SOD2-H₂O₂pathway. Oncotarget. 8:3344–3354. 2017. View Article : Google Scholar : PubMed/NCBI
2	Rosebush MS, Rao SK, Samant S, Gu W, Handorf CR, Pfeffer LM and Nosrat CA: Oral cancer: Enduring characteristics and emerging trends. J Mich Dent Assoc. 94:64–68. 2012.PubMed/NCBI
3	Chen C, Méndez E, Houck J, Fan W, Lohavanichbutr P, Doody D, Yueh B, Futran ND, Upton M, Farwell DG, et al: Gene expression profiling identifies genes predictive of oral squamous cell carcinoma. Cancer Epidemiol Biomarkers Prev. 17:2152–2162. 2008. View Article : Google Scholar : PubMed/NCBI
4	Fang Z, Wu L, Wang L, Yang Y, Meng Y and Yang H: Increased expression of the long non-coding RNA UCA1 in tongue squamous cell carcinomas: A possible correlation with cancer metastasis. Oral Surg Oral Med Oral Pathol Oral Radiol. 117:89–95. 2014. View Article : Google Scholar : PubMed/NCBI
5	Valencia-Sanchez MA, Liu J, Hannon GJ and Parker R: Control of translation and mRNA degradation by miRNAs and siRNAs. Genes Dev. 20:515–524. 2006. View Article : Google Scholar : PubMed/NCBI
6	Pillai RS, Bhattacharyya SN and Filipowicz W: Repression of protein synthesis by miRNAs: How many mechanisms? Trends Cell Biol. 17:118–126. 2007. View Article : Google Scholar : PubMed/NCBI
7	Yu J, Ryan DG, Getsios S, Oliveira-Fernandes M, Fatima A and Lavker RM: MicroRNA-184 antagonizes microRNA-205 to maintain SHIP2 levels in epithelia. Proc Natl Acad Sci USA. 105:19300–19305. 2008. View Article : Google Scholar : PubMed/NCBI
8	Ambros V: The functions of animal microRNAs. Nature. 431:350–355. 2004. View Article : Google Scholar : PubMed/NCBI
9	Tung MC, Lin PL, Cheng YW, Wu DW, Yeh SD, Chen CY and Lee H: Reduction of microRNA-184 by E6 oncoprotein confers cisplatin resistance in lung cancer via increasing Bcl-2. Oncotarget. 7:32362–32374. 2016. View Article : Google Scholar : PubMed/NCBI
10	Emdad L, Janjic A, Alzubi MA, Hu B, Santhekadur PK, Menezes ME, Shen XN, Das SK, Sarkar D and Fisher PB: Suppression of miR-184 in malignant gliomas upregulates SND1 and promotes tumor aggressiveness. Neuro Oncol. 17:419–429. 2015. View Article : Google Scholar : PubMed/NCBI
11	Foley NH, Bray IM, Tivnan A, Bryan K, Murphy DM, Buckley PG, Ryan J, O'Meara A, O'Sullivan M and Stallings RL: MicroRNA-184 inhibits neuroblastoma cell survival through targeting the serine/threonine kinase AKT2. Mol Cancer. 9:832010. View Article : Google Scholar : PubMed/NCBI
12	Zhen Y, Liu Z, Yang H, Yu X, Wu Q, Hua S, Long X, Jiang Q, Song Y, Cheng C, et al: Tumor suppressor PDCD4 modulates miR-184-mediated direct suppression of C-MYC and BCL2 blocking cell growth and survival in nasopharyngeal carcinoma. Cell Death Dis. 4:e8722013. View Article : Google Scholar : PubMed/NCBI
13	Wu GG, Li WH, He WG, Jiang N, Zhang GX, Chen W, Yang HF, Liu QL, Huang YN, Zhang L, et al: Mir-184 post-transcriptionally regulates SOX7 expression and promotes cell proliferation in human hepatocellular carcinoma. PLoS One. 9:e887962014. View Article : Google Scholar : PubMed/NCBI
14	Wong TS, Liu XB, Wong BY, Ng RW, Yuen AP and Wei WI: Mature miR-184 as potential oncogenic microRNA of squamous cell carcinoma of tongue. Clin Cancer Res. 14:2588–2592. 2008. View Article : Google Scholar : PubMed/NCBI
15	Lefebvre V, Dumitriu B, Penzo-Méndez A, Han Y and Pallavi B: Control of cell fate and differentiation by Sry-related high-mobility-group box (Sox) transcription factors. Int J Biochem Cell Biol. 39:2195–2214. 2007. View Article : Google Scholar : PubMed/NCBI
16	Liu H, Mastriani E, Yan ZQ, Yin SY, Zeng Z, Wang H, Li QH, Liu HY, Wang X, Bao HX, et al: SOX7 co-regulates Wnt/β-catenin signaling with Axin-2: Both expressed at low levels in breast cancer. Sci Rep. 6:261362016. View Article : Google Scholar : PubMed/NCBI
17	Harley VR, Lovell-Badge R and Goodfellow PN: Definition of a consensus DNA binding site for SRY. Nucleic Acids Res. 22:1500–1501. 1994. View Article : Google Scholar : PubMed/NCBI
18	Stovall DB, Cao P and Sui G: SOX7: From a developmental regulator to an emerging tumor suppressor. Histol Histopathol. 29:439–445. 2014.PubMed/NCBI
19	Zheng Z, Liu J, Yang Z, Wu L, Xie H, Jiang C, Lin B, Chen T, Xing C, Liu Z, et al: MicroRNA-452 promotes stem-like cells of hepatocellular carcinoma by inhibiting Sox7 involving Wnt/β-catenin signaling pathway. Oncotarget. 7:28000–28012. 2016. View Article : Google Scholar : PubMed/NCBI
20	Yu X and Li Z: MicroRNA expression and its implications for diagnosis and therapy of tongue squamous cell carcinoma. J Cell Mol Med. 20:10–16. 2016. View Article : Google Scholar : PubMed/NCBI
21	Calin GA and Croce CM: MicroRNA signatures in human cancers. Nat Rev Cancer. 6:857–866. 2006. View Article : Google Scholar : PubMed/NCBI
22	Shiomi N, Mori M, Tsuji H, Imai T, Inoue H, Tateishi S, Yamaizumi M and Shiomi T: Human RAD18 is involved in S phase-specific single-strand break repair without PCNA monoubiquitination. Nucleic Acids Res. 35:e92007. View Article : Google Scholar : PubMed/NCBI
23	Deng J, He M, Chen L, Chen C, Zheng J and Cai Z: The loss of miR-26a-mediated post-transcriptional regulation of cyclin E2 in pancreatic cancer cell proliferation and decreased patient survival. PLoS One. 8:e764502013. View Article : Google Scholar : PubMed/NCBI
24	Shen F, Cai WS, Feng Z, Li JL, Chen JW, Cao J and Xu B: MiR-492 contributes to cell proliferation and cell cycle of human breast cancer cells by suppressing SOX7 expression. Tumour Biol. 36:1913–1921. 2015. View Article : Google Scholar : PubMed/NCBI
25	Liu H, Yan ZQ, Li B, Yin SY, Sun Q, Kou JJ, Ye D, Ferns K, Liu HY and Liu SL: Reduced expression of SOX7 in ovarian cancer: A novel tumor suppressor through the Wnt/β-catenin signaling pathway. J Ovarian Res. 7:872014. View Article : Google Scholar : PubMed/NCBI
26	Yang M, Cui G, Ding M, Yang W, Liu Y, Dai D and Chen L: miR-935 promotes gastric cancer cell proliferation by targeting SOX7. Biomed Pharmacother. 79:153–158. 2016. View Article : Google Scholar : PubMed/NCBI
27	Man CH, Fung TK, Wan H, Cher CY, Fan A, Ng N, Ho C, Wan TS, Tanaka T, So CW, et al: Suppression of SOX7 by DNA methylation and its tumor suppressor function in acute myeloid leukemia. Blood. 125:3928–3936. 2015. View Article : Google Scholar : PubMed/NCBI
28	Schepers GE, Teasdale RD and Koopman P: Twenty pairs of sox: Extent, homology, and nomenclature of the mouse and human sox transcription factor gene families. Dev Cell. 3:167–170. 2002. View Article : Google Scholar : PubMed/NCBI