Open Access

Identification of common differentially‑expressed miRNAs in ovarian cancer cells and their exosomes compared with normal ovarian surface epithelial cell cells

  • Authors:
    • Shitao Zhang
    • Xiaoping Zhang
    • Xueqi Fu
    • Wannan Li
    • Shu Xing
    • Yiling Yang
  • View Affiliations

  • Published online on: June 12, 2018     https://doi.org/10.3892/ol.2018.8954
  • Pages: 2391-2401
  • Copyright: © Zhang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

The aim of the present study was to identify common microRNAs (miRNAs) in ovarian cancer (OC) cells and their exosomes using microarray data (accession number GSE76449) available from the Gene Expression Omnibus database, including exosomal samples from 3 OC cell lines, 1 normal ovarian surface epithelial cell line and their original cell samples. Differentially‑expressed miRNAs (DE‑miRNAs) were identified using the Linear Models for Microarray data method, and mRNA targets of DE‑miRNAs were predicted using the miRWalk2 database. The potential functions of the target genes of the DE‑miRNAs were analyzed using the Database for Annotation, Visualization and Integrated Discovery tool. The association between crucial miRNAs and target genes, and their clinical associations, were validated using The Cancer Genome Atlas data. As a result, 12 upregulated and 12 downregulated DE‑miRNAs were shared by the 3 OC cell lines compared with normal controls in the exosomal samples, while 5 upregulated and 65 downregulated DE‑miRNAs were shared between the original cells. Among them, 9 downregulated DE‑miRNAs were shared between exosomal and original cells. The target genes of 4 common DE‑miRNAs between exosomal and original cells (miR‑127‑3p, miR‑339‑5p, miR‑409‑3p and miR‑654‑3p) were predicted. Functional enrichment analysis indicated that these target genes may be involved in the Wnt signaling pathway (miR‑409‑3p‑CTBP1 and miR‑339‑5p‑CHD8) and Proteoglycans in cancer (miR‑127‑3p‑PPP1CA). The negative associations between these 3 miRNAs and target genes were confirmed by a Pearson's correlation analysis. miR‑127 was negatively associated with tumor grade. In conclusion, our results describe a set of miRNAs involved in OC development, in exosomal and non‑exosomal manners, by regulating their target genes. They may be potential targets for treatment of OC.
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August-2018
Volume 16 Issue 2

Print ISSN: 1792-1074
Online ISSN:1792-1082

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Copy and paste a formatted citation
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Spandidos Publications style
Zhang S, Zhang X, Fu X, Li W, Xing S and Yang Y: Identification of common differentially‑expressed miRNAs in ovarian cancer cells and their exosomes compared with normal ovarian surface epithelial cell cells. Oncol Lett 16: 2391-2401, 2018
APA
Zhang, S., Zhang, X., Fu, X., Li, W., Xing, S., & Yang, Y. (2018). Identification of common differentially‑expressed miRNAs in ovarian cancer cells and their exosomes compared with normal ovarian surface epithelial cell cells. Oncology Letters, 16, 2391-2401. https://doi.org/10.3892/ol.2018.8954
MLA
Zhang, S., Zhang, X., Fu, X., Li, W., Xing, S., Yang, Y."Identification of common differentially‑expressed miRNAs in ovarian cancer cells and their exosomes compared with normal ovarian surface epithelial cell cells". Oncology Letters 16.2 (2018): 2391-2401.
Chicago
Zhang, S., Zhang, X., Fu, X., Li, W., Xing, S., Yang, Y."Identification of common differentially‑expressed miRNAs in ovarian cancer cells and their exosomes compared with normal ovarian surface epithelial cell cells". Oncology Letters 16, no. 2 (2018): 2391-2401. https://doi.org/10.3892/ol.2018.8954