ARHGAP24 regulates cell ability and apoptosis ofcolorectal cancer cells via the regulation of P53

Zhang,Suiliang; Sui,Liang; Zhuang,Juhua; He,Saifei; Song,Yanan; Ye,Ying; Xia,Wei

doi:10.3892/ol.2018.9075

ARHGAP24 regulates cell ability and apoptosis ofcolorectal cancer cells via the regulation of P53

Authors:
- Suiliang Zhang
- Liang Sui
- Juhua Zhuang
- Saifei He
- Yanan Song
- Ying Ye
- Wei Xia
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Affiliations: Department of Oncology and Interventional Therapy, The Seventh People's Hospital of Shanghai University of Traditional Chinese Medicine, Shanghai 200137, P.R. China, Department of Emergency, Ruijin Hospital, Medical School of Shanghai Jiaotong University, Shanghai 200025, P.R. China, Department of Nuclear Medicine, The Seventh People's Hospital of Shanghai University of Traditional Chinese Medicine, Shanghai 200137, P.R. China
Published online on: July 4, 2018 https://doi.org/10.3892/ol.2018.9075
Pages: 3517-3524

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Abstract

Colorectal cancer is a human malignancy ranked the third highest of the global incidence of malignant tumors. Rho GTPase‑activating proteins (RHOGAPs) were identified functional in several processes of tumors. In the present study, through reverse transcription-quantitative PCR (RT-qPCR) and western blot analysis, expression of Rho GTPase‑activating protein 24 (ARHGAP24) and p53 was measured in colorectal cancer tissues, which was lower than that in adjacent normal tissues, revealing that ARHGAP24 may be implicated in the progress of colorectal cancer and in vitro, overexpression of ARHGAP24 in LoVo and HCT116 cells inhibited the cell ability and enhanced cell apoptosis, and accompanied with high protein expression of p53, p21 and Bax. Further, addition of p53 inhibitor PFT-α had an antagonistic effect on cell proliferation and apoptosis of LoVo and HCT116 cells induced by ARHGAP24 overexpression. In addition, the expression of p21 and Bax was positively correlated with p53 expression. All of the above data demonstrated that ARHGAP24 was likely to be a tumor suppressor in colorectal cancer and may function closely related to p53, p21 and Bax. We inferred that ARHGAP24 may be a novel target for in-depth study of colorectal cancer.

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