Protective effect and mechanism of rat recombinant S100 calcium‑binding protein A4 on oxidative stress injury of rat vascular endothelial cells

Meng,Xiangyan; Gao,Xiujie; Zhang,Zhiqing; Zhou,Xuesi; Wu,Lei; Yang,Miaomiao; Wang,Kun; Ren,Hanlin; Sun,Bei; Wang,Tianhui

doi:10.3892/ol.2018.9135

Protective effect and mechanism of rat recombinant S100 calcium‑binding protein A4 on oxidative stress injury of rat vascular endothelial cells

Authors:
- Xiangyan Meng
- Xiujie Gao
- Zhiqing Zhang
- Xuesi Zhou
- Lei Wu
- Miaomiao Yang
- Kun Wang
- Hanlin Ren
- Bei Sun
- Tianhui Wang
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Affiliations: Performance Medicine Laboratory, Department of Performance Medicine, Tianjin Institute of Health and Environmental Medicine, Tianjin 300050, P.R. China, Key Laboratory of Hormones and Development (Ministry of Health), Department of Physiology, Metabolic Diseases Hospital and Tianjin Institute of Endocrinology, Tianjin Medical University, Tianjin 300070, P.R. China
Published online on: July 11, 2018 https://doi.org/10.3892/ol.2018.9135
Pages: 3614-3622
Copyright: © Meng et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

The aim of the present study was to examine the protective effects and mechanisms of S100 calcium‑binding protein A4 (S100A4) on endothelial cell apoptosis induced by oxidative stress injury. Endothelial cells were cultured and divided into control and oxidative stress injury groups, with the latter state induced by H2O2. Endothelial cells in every group were incubated with or without 50 or 100 µM S100A4. The cell viability and amounts of malondialdehyde, nitric oxide and lactate dehydrogenase in the culture medium were measured. The apoptotic index was detected by TUNEL staining. Western blot and immunoprecipitation analyses were used to detect the expression levels and the association between S100A4 and P53. H2O2 treatment led to oxidative stress injury in the cultured vascular endothelial cells, a decrease in the cell viability and an increase in the rate of apoptosis of vascular endothelial cells compared with the negative control group. Exogenous S100A4 serves a significant function against oxidative stress injury (P<0.05), increasing the viability and attenuating the apoptotic rate of endothelial cells. Western blotting results suggested that the protein levels of S100A4 and P53 increased subsequent to oxidative stress injury and that exogenous S100A4 increased the expression of P53 in the cytoplasm and decreased the expression of P53 in nucleus. The immunoprecipitation assay results revealed a protein‑protein interaction between S100A4 and P53. These results suggested that rat recombinant S100A4 serves an anti‑apoptotic function in oxidative stress injury. This effect of S100A4 is mediated, at least in part, via the inhibition of the translocation of P53 to the nucleus.

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