Mechanism of the enhancing effects of miR‑93 on resistance of breast cancer MCF‑7 cells to adriamycin
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- Published online on: June 29, 2018 https://doi.org/10.3892/ol.2018.9054
- Pages: 3779-3783
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Copyright: © Wang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
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Abstract
This study aimed to investigate the effects of miR‑93 on resistance of breast cancer MCF‑7 cells to adriamycin, and to explore the possible mechanism. Expression of miR‑93 in breast cancer cell lines MCF‑7 and MCF‑7/ADM was detected by reverse transcription-quantitative PCR (RT‑qPCR). miR‑93 mimics and inhibitors were transfected into MCF‑7/ADM and MCF‑7 cells, and MTT assay was used to detect the resistance of cells to adriamycin after transfection. Western blot analysis was used to detect the expression of anti‑apoptotic protein Bcl‑2 and multidrug resistance gene MDR1 related P‑gp protein in MCF‑7/ADM and MCF‑7 cells before and after the transfection of miR‑93 mimics. Expression level of miR‑93 in MCF‑7/ADM cells was decreased, and was 40% of that in MCF‑7 cells (0.39±0.04, p<0.05). Before transfection, IC50 value of MCF‑7 cells to adriamycin (11.02±0.95) was lower than that of MCF‑7/ADM cells (21.29±1.83, p<0.05). IC50 value of MCF‑7/ADR cells at 72 h after transfection with miR‑93 mimics (13.55±0.86) was lower than that of the negative control group (24.67±1.51, p<0.05). IC50 value of MCF‑7 cells 72 h after transfection with miR‑93 inhibitor (19.88±1.28) was higher than that of negative control group (11.02±0.95, p<0.05). Expression levels of Bcl‑2 and P‑gp proteins in MCF‑7/ADM cells were 1.63±0.24 and 1.76±0.22 times that of MCF‑7 cells, respectively (p<0.05). At 72 h after transfection of miR‑93 mimics, expression levels of Bcl‑2 and P‑gp proteins in MCF‑7/ADM cells were 0.27±0.06 and 0.39±0.05, respectively, compared with the negative control group (p<0.05). At 72 h after transfection with miR‑93 inhibitor, expression levels of Bcl‑2 and P‑gp protein in MCF‑7 cells were 1.48±0.10 and 1.56±0.11 times of the negative control group, respectively (p<0.05). miR‑93 can increase the apoptosis of MCF‑7/ADM cells and their resistance to adriamycin by inhibiting the expression of Bcl‑2 and P‑gp proteins.
