Regulation of miR‑155 affects the invasion and migration of gastric carcinoma cells by modulating the STAT3 signaling pathway

Wei,Hua; Li,Yan; Ning,Qiang; Suo,Zhi‑Min

doi:10.3892/ol.2018.9152

Regulation of miR‑155 affects the invasion and migration of gastric carcinoma cells by modulating the STAT3 signaling pathway

Authors:
- Hua Wei
- Yan Li
- Qiang Ning
- Zhi‑Min Suo
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Affiliations: Department of Endoscopy, Huaihe Hospital Affiliated to Henan University, Kaifeng, Henan 475000, P.R. China, Department of Gastroenterology, Huaihe Hospital Affiliated to Henan University, Kaifeng, Henan 475000, P.R. China, Department of Endoscopy, Third Hospital of Wafangdian, Dalian, Liaoning 116300, P.R. China
Published online on: July 16, 2018 https://doi.org/10.3892/ol.2018.9152
Pages: 4137-4142
Copyright: © Wei et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Studies investigating the effects of microRNA (miR)‑155 on the behavior of tumor cells have concentrated primarily on proliferation and apoptosis. The aim of the present study was to investigate the effect of miR‑155 inhibitor on the metastatic and invasive ability of gastric carcinoma cells and whether this effect is mediated via the signal transduction and activators of transcription 3 (STAT3) signaling pathway. The miR‑155 inhibitor and miR‑155 negative control (NC) were transfected into the AGs and MKN‑45 cell lines. The migratory and invasive abilities of the cells were analyzed. The level of phosphorylated (p‑)STAT3 and the expression levels of matrix metalloproteinases (MMPs), vascular endothelial growth factor (VEGF) and suppressor of cytokine signaling 1 (SOCS1) were also detected. For the AGS cell line, the cell counts (mean ± standard deviation) for the Transwell migration assay were 98.99±9.13 in the miR‑155 NC group and 45.32±4.32 in the miR‑155 inhibitor group (P<0.01). For the MKN‑45 cell line, the cell counts for the migration assay were 129.99±10.12 and 50.36±5.2 in the miR‑155 NC and miR‑155 inhibitor groups, respectively (P<0.01). The cell counts of the AGS cell line for the invasion assay were 70.25±7.94 in the miR‑155 NC group and 40.68±4.73 in the miR‑155 inhibitor group (P<0.05). For the MKN‑45 cell line, the cell counts for the invasion assay were 84.63±8.12 and 40.35±4.29 in the miR‑155 NC and miR‑155 inhibitor groups, respectively (P<0.05). Transfection with the miR‑155 inhibitor was able to significantly decrease the level of p‑STAT3 in the AGS and MKN‑45 cell lines compared with the negative control group (all P<0.05). The levels of MMP2 and MMP9 expression were decreased following transfection with miR‑155 in AGS and MKN‑45 cells (both P<0.05). Notably, transfection with the miR‑155 inhibitor was able to decrease the level of VEGF expression, whilst increasing the SOCS1 expression level compared with the negative control group (both P<0.05). Additionally, the downregulation of miR‑155 expression in gastric carcinoma cell lines was able to significantly decrease the expression of VEGF, MMP2 and MMP9, thereby inhibiting the invasion and metastasis of gastric carcinoma cells.

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