The expression level of CSDAP1 in lung cancer and its clinical significance

Xu,Tongbai; Li,Dongsheng; He,Yuan; Zhang,Fuliang; Qiao,Man; Chen,Yanhua

doi:10.3892/ol.2018.9195

The expression level of CSDAP1 in lung cancer and its clinical significance

Authors:
- Tongbai Xu
- Dongsheng Li
- Yuan He
- Fuliang Zhang
- Man Qiao
- Yanhua Chen
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Affiliations: Department of Respiratory Medicine, Tianjin Nankai Hospital, Tianjin 300100, P.R. China
Published online on: July 23, 2018 https://doi.org/10.3892/ol.2018.9195
Pages: 4361-4366

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Abstract

Expression level of messenger RNA (mRNA) of cold shock domain protein A intronless pseudogene (CSDAP1) in lung cancer tissues was studied. Fresh pathological specimens collected from 317 patients with primary lung cancer through surgical resection from January 2007 to January 2012 were selected. Reverse transcription-polymerase chain reaction (RT-PCR) and western blot analysis were used to detect the transcription and translation of CSDAP1 in lung cancer tissues and cancer-adjacent normal tissues, and the results were analyzed in combination with clinicopathological features and prognosis of lung cancer. Among 317 lung cancer specimens, 105 cases (33.1%) had high expression of CSDAP1. Among 138 cases of pulmonary adenocarcinoma, 59 cases had high expression of CSDAP1, and the high expression rate was 42.8%. Among 170 cases of lung squamous cell carcinoma, 46 cases had high expression of CSDAP1, and the high expression rate was 27.5% (P<0.05). Three cases of large cell carcinoma and 9 cases of small cell carcinoma had extremely low expression or had no expression of CSDAP1. Among the 127 lung cancer patients with regional lymph node metastasis, 53 cases (41.7%) had high expression of CSDAP1, while among the 190 lung cancer patients without regional lymph node metastasis, 52 cases (27.4%) had high expression of CSDAP1 (P<0.05). The results also revealed that the expression of CSDAP1 was also related to tumor-node-metastasis (TNM) staging of lung cancer. One-year, three-year and five-year survival rates of lung cancer patients who had no expression of CSDAP1 were relatively high (P<0.05). The results suggested that CSDAP1 may play an important role in the occurrence, development and judgement of prognosis of lung cancer.

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1	Tang H, Wang S, Xiao G, Schiller J, Papadimitrakopoulou V, Minna J, Wistuba II and Xie Y: Comprehensive evaluation of published gene expression prognostic signatures for biomarker-based lung cancer clinical studies. Ann Oncol. 28:733–740. 2017. View Article : Google Scholar : PubMed/NCBI
2	Xu C, Xie S, Song C, Huang L and Jiang Z: Lin28 mediates cancer chemotherapy resistance via regulation of miRNA signaling. Hepatogastroenterology. 61:1138–1141. 2014.PubMed/NCBI
3	De Martino M, Forzati F, Arra C, Fusco A and Esposito F: HMGA1-pseudogenes and cancer. Oncotarget. 7:28724–28735. 2016. View Article : Google Scholar : PubMed/NCBI
4	Lister N, Shevchenko G, Walshe JL, Groen J, Johnsson P, Vidarsdóttir L, Grander D, Ataide SF and Morris KV: The molecular dynamics of long noncoding RNA control of transcription in PTEN and its pseudogene. Proc Natl Acad Sci USA. 114:9942–9947. 2017. View Article : Google Scholar : PubMed/NCBI
5	Harrison PM, Zheng D, Zhang Z, Carriero N and Gerstein M: Transcribed processed pseudogenes in the human genome: An intermediate form of expressed retrosequence lacking protein-coding ability. Nucleic Acids Res. 33:2374–2383. 2005. View Article : Google Scholar : PubMed/NCBI
6	Livak KJ and Schmittgen TD: Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) Method. METHODS. 25:402–408. 2001. View Article : Google Scholar : PubMed/NCBI
7	Zhu YJ, Zhang HB, Liu YH, Zhu YZ, Chen J, Li Y, Bai JP, Liu LR, Qu YC, Qu X, et al: Association of mutant EGFR L858R and exon 19 concentration in circulating cell-free DNA using droplet digital PCR with response to EGFR-TKIs in NSCLC. Oncol Lett. 14:2573–2579. 2017. View Article : Google Scholar : PubMed/NCBI
8	Wang X, Che H, Zhang W, Wang J, Ke T, Cao R, Meng S, Li D, Weiming O, Chen J, et al: Effects of mild chronic intermittent cold exposure on rat organs. Int J Biol Sci. 11:1171–1180. 2015. View Article : Google Scholar : PubMed/NCBI
9	Dal Piaz F, Terracciano S, De Tommasi N and Braca A: Hsp90 activity modulation by plant secondary metabolites. Planta Med. 81:1223–1239. 2015. View Article : Google Scholar : PubMed/NCBI
10	Kanshin E, Kubiniok P, Thattikota Y, D'Amours D and Thibault P: Phosphoproteome dynamics of Saccharomyces cerevisiae under heat shock and cold stress. Mol Syst Biol. 11:8132015. View Article : Google Scholar : PubMed/NCBI
11	Tanabe Y, Nagatoishi S and Tsumoto K: Thermodynamic characterization of the interaction between the human Y-box binding protein YB-1 and nucleic acids. Mol Biosyst. 11:2441–2448. 2015. View Article : Google Scholar : PubMed/NCBI
12	Cai CJ, Ye SJ, Zhu W, Sun LY, Wan HS, Feng ZH, Ma L and Li L: Study on invasion and metastasis-associated genes of lung cancer related with NM23-H1 gene. Sichuan Da Xue Xue Bao Yi Xue Ban. 41:941–945. 2010.(In Chinese). PubMed/NCBI
13	Diamond P, Shannon MF, Vadas MA and Coles LS: Cold shock domain factors activate the granulocyte-macrophage colony-stimulating factor promoter in stimulated Jurkat T cells. J Biol Chem. 276:7943–7951. 2001. View Article : Google Scholar : PubMed/NCBI
14	Tang C, Wang Y, Lan D, Feng X, Zhu X, Nie P and Yue H: Analysis of gene expression profiles reveals the regulatory network of cold-inducible RNA-binding protein mediating the growth of BHK-21 cells. Cell Biol Int. 39:678–689. 2015. View Article : Google Scholar : PubMed/NCBI
15	Kalamida D, Karagounis IV, Mitrakas A, Kalamida S, Giatromanolaki A and Koukourakis MI: Fever-range hyperthermia vs. hypothermia effect on cancer cell viability, proliferation and HSP90 expression. PLoS One. 10:e01160212015. View Article : Google Scholar : PubMed/NCBI
16	Hung MS, Lin YC, Mao JH, Kim IJ, Xu Z, Yang CT, Jablons DM and You L: Functional polymorphism of the CK2alpha intronless gene plays oncogenic roles in lung cancer. PLoS One. 5:e114182010. View Article : Google Scholar : PubMed/NCBI
17	Chen SJ, Lin PW, Lin HP, Huang SS, Lai FJ, Sheu HM, Hsu LJ and Chang NS: UV irradiation/cold shock-mediated apoptosis is switched to bubbling cell death at low temperatures. Oncotarget. 6:8007–8018. 2015.PubMed/NCBI
18	Yamashita T, Higashi M, Momose S, Morozumi M and Tamaru JI: Nuclear expression of Y box binding-1 is important for resistance to chemotherapy including gemcitabine in TP53-mutated bladder cancer. Int J Oncol. 51:579–586. 2017. View Article : Google Scholar : PubMed/NCBI
19	Zimmermann M, Traxler D, Simader E, Bekos C, Dieplinger B, Lainscak M, Ankersmit HJ and Mueller T: In vitro stability of heat shock protein 27 in serum and plasma under different pre-analytical conditions: Implications for large-scale clinical studies. Ann Lab Med. 36:353–357. 2016. View Article : Google Scholar : PubMed/NCBI
20	Liu Y, Hu W, Murakawa Y, Yin J, Wang G, Landthaler M and Yan J: Cold-induced RNA-binding proteins regulate circadian gene expression by controlling alternative polyadenylation. Sci Rep. 3:20542013. View Article : Google Scholar : PubMed/NCBI
21	Yu B, Wang J, He C, Wang W, Tang J, Zheng R, Zhou C, Zhang H, Fu Z, Li Q, et al: Cytokine-induced killer cell therapy for modulating regulatory T cells in patients with non-small cell lung cancer. Exp Ther Med. 14:831–840. 2017. View Article : Google Scholar : PubMed/NCBI