Long non‑coding RNA‑CCAT2 promotes the occurrence of non‑small cell lung cancer by regulating the Wnt/β‑catenin signaling pathway

Zhao,Chengling; Qiao,Chenchen; Zong,Liguo; Chen,Yuqing

doi:10.3892/ol.2018.9194

Long non‑coding RNA‑CCAT2 promotes the occurrence of non‑small cell lung cancer by regulating the Wnt/β‑catenin signaling pathway

Authors:
- Chengling Zhao
- Chenchen Qiao
- Liguo Zong
- Yuqing Chen
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Affiliations: School of Medicine, Shandong University School of Medicine, Jinan, Shandong 250100, P.R. China, Department of Cardiology, First Municipal Hospital of Bengbu, Bengbu, Anhui 233004, P.R. China, Department of Intensive Care Unit, Zaozhuang Municipal Hospital, Zaozhuang, Shandong 277001, P.R. China, Anhui Clinical and Preclinical Key Laboratory of Respiratory Diseases, Department of Respiration, First Affiliated Hospital of Bengbu Medical College, Bengbu, Anhui 233004, P.R. China
Published online on: July 23, 2018 https://doi.org/10.3892/ol.2018.9194
Pages: 4600-4606

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Abstract

The present study aimed to investigate the biological function of colon cancer‑associated transcript 2 (CCAT2) in the occurrence and progression of non‑small cell lung carcinoma (NSCLC) and its potential use in the early diagnosis and molecular‑targeted therapy of NSCLC. The tumor tissues, para‑carcinoma tissues and associated clinical data of 36 patients with NSCLC were collected in order to detect the expression of CCAT2 and assess the impact of factors including histopathological type, Tumor‑Node‑Metastasis stage and lymph node metastasis on CCAT2 expression. The lung cancer NCI‑H1975 cell line was transfected with a small interfering RNA (siRNA) plasmid to determine the effect of si‑CCAT2 on NSCLC proliferation, invasion and metastasis. The effect of si‑CCAT2 on the expression of nuclear and cytoplasmic β‑catenin protein in the lung cancer NCI‑H1975 cell line was detected using western blot analysis. The expression levels of CCAT2 in the tumor tissues of patients with NSCLC were significantly higher than those in the normal para‑carcinoma tissues (t=8.580, P<0.01). Subsequent to CCAT2 silencing, the proliferation and invasive abilities of NCI‑H1975 cells were significantly decreased compared with control cells (P<0.05). In the si‑CCAT2 group, the level of nuclear and cytoplasmic β‑catenin proteins was decreased, and the activity of the Wnt signaling pathway was significantly inhibited compared with the control cells (P<0.01), and a synergistic effect was exerted with the Wnt signaling inhibitor FH535. CCAT2 may therefore promote the occurrence of NSCLC by regulating the Wnt/β‑catenin signaling pathway.

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