Open Access

Regulating glioma stem cells by hypoxia through the Notch1 and Oct3/4 signaling pathway

  • Authors:
    • Fei Zeng
    • Hong Chen
    • Zhaohui Zhang
    • Tao Yao
    • Guan Wang
    • Qingxing Zeng
    • Shenhan Duan
    • Yanqiang Zhan
  • View Affiliations

  • Published online on: September 17, 2018     https://doi.org/10.3892/ol.2018.9442
  • Pages: 6315-6322
  • Copyright: © Zeng et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

To investigate the effects of hypoxia on the features of cancer stem cells in the glioma cancer U87 cell line and underlying mechanism, stem cell markers and features in U87 were studied under the hypoxic and normoxic culture conditions by reverse transcription‑quantitative polymerase chain reaction, western blot analysis, MTT, a colony formation test and flow cytometry. Compared to the normoxic group, the cluster of differentiation 133+ phenotype, clone formation rate and cell vitality were significantly elevated in U87 cells cultured in a hypoxic microenvironment. Also, the mRNA and protein expression of neurogenic locus notch homolog protein 1 (Notch1) and Oct3/4 were significantly elevated in U87 cells cultured in a hypoxic microenvironment, however, transcription factor SOX‑2 expression was not significantly changed. These results indicate that hypoxia can promote the proliferation of glioma stem cells and maintain the characteristics of stem cells through the activation of Notch1 and Oct3/4 or Notch1 activation, affecting the biological characteristics of glioma cells.
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November-2018
Volume 16 Issue 5

Print ISSN: 1792-1074
Online ISSN:1792-1082

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Spandidos Publications style
Zeng F, Chen H, Zhang Z, Yao T, Wang G, Zeng Q, Duan S and Zhan Y: Regulating glioma stem cells by hypoxia through the Notch1 and Oct3/4 signaling pathway. Oncol Lett 16: 6315-6322, 2018.
APA
Zeng, F., Chen, H., Zhang, Z., Yao, T., Wang, G., Zeng, Q. ... Zhan, Y. (2018). Regulating glioma stem cells by hypoxia through the Notch1 and Oct3/4 signaling pathway. Oncology Letters, 16, 6315-6322. https://doi.org/10.3892/ol.2018.9442
MLA
Zeng, F., Chen, H., Zhang, Z., Yao, T., Wang, G., Zeng, Q., Duan, S., Zhan, Y."Regulating glioma stem cells by hypoxia through the Notch1 and Oct3/4 signaling pathway". Oncology Letters 16.5 (2018): 6315-6322.
Chicago
Zeng, F., Chen, H., Zhang, Z., Yao, T., Wang, G., Zeng, Q., Duan, S., Zhan, Y."Regulating glioma stem cells by hypoxia through the Notch1 and Oct3/4 signaling pathway". Oncology Letters 16, no. 5 (2018): 6315-6322. https://doi.org/10.3892/ol.2018.9442