Expression pattern and level of ING5 protein in normal and cancer tissues

Yang,Xue‑Feng; Shen,Dao‑Fu; Zhao,Shuang; Ren,Tian‑Ren; Gao,Yang; Shi,Shuai; Wu,Ji‑Cheng; Sun,Hong‑Zhi; Zheng,Hua‑Chuan

doi:10.3892/ol.2018.9581

Expression pattern and level of ING5 protein in normal and cancer tissues

Authors:
- Xue‑Feng Yang
- Dao‑Fu Shen
- Shuang Zhao
- Tian‑Ren Ren
- Yang Gao
- Shuai Shi
- Ji‑Cheng Wu
- Hong‑Zhi Sun
- Hua‑Chuan Zheng
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Affiliations: Cancer Center and Key Laboratory of Brain and Spinal Cord Injury of Liaoning Province, The First Affiliated Hospital of Jinzhou Medical University, Jinzhou, Liaoning 121001, P.R. China, Jilin Province Forestry Bureau, Linjiang, Jilin 134600, P.R. China
Published online on: October 16, 2018 https://doi.org/10.3892/ol.2018.9581
Pages: 63-68
Copyright : © Yang et al. This is an open access article distributed under the terms of Creative Commons Attribution License [CC BY 4.0].

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Abstract

Inhibitor of growth family 5 (ING5) functions as a type‑II tumor suppressor gene and exerts an important role in DNA repair, apoptotic induction, proliferative inhibition, chromatin remodeling and the invasion process. In the present study, immunohistochemistry was performed to characterize the expression profile of ING5 protein on a tissue microarray containing mouse and human normal tissues, and human cancer tissues, including hepatocellular (n=62), renal clear cell (n=62), pancreatic (n=62), esophageal squamous cell (n=45), cervical squamous cell (n=31), breast (n=144), gastric (n=196), colorectal (n=96), endometrial (n=96) and lung carcinoma (n=192). In the mouse tissues, ING5 expression was detected in the cytoplasm of neurons, the nephric tubule and glomerulus, alveolar epithelium, gastrointestinal glands, squamous epithelium of the skin and skeletal muscles. By contrast, ING5 was localized to the cell nucleus in breast tissues. In human tissues, ING5 protein was primarily localized in the cytoplasm. However, ING5 was detected in the cytoplasm and nucleus in various types of normal tissues, including the tongue, stomach, intestine, lung and breast. In total, ING5 expression was detected in 400/986 cancer tissues (40.6%). In the majority of cases, ING5 expression was observed to be restricted to the cytoplasm. However, ING5 was also detected in the nucleus in a number of cancer tissues, including gastric, colorectal and lung carcinoma. Notably, ING5 was more frequently expressed in breast (79.9%), colorectal (56.3%) and endometrial carcinoma (50.0%). The incidence of ING5 expression in hepatocellular carcinoma (14.5%) and pancreatic carcinoma (22.6%) was low. These findings indicate that ING5 may be involved in cell regeneration and be associated with colorectal carcinogenesis.

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