Rhamnetin induces apoptosis in human breast cancer cells via the miR‑34a/Notch‑1 signaling pathway

  • Authors:
    • Lan Lan
    • Yue Wang
    • Zhanyu Pan
    • Bin Wang
    • Zhensong Yue
    • Zhansheng Jiang
    • Ling Li
    • Cong Wang
    • Hongmei Tang
  • View Affiliations

  • Published online on: October 15, 2018     https://doi.org/10.3892/ol.2018.9575
  • Pages: 676-682
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Abstract

The present study aimed to investigate whether rhamnetin induced apoptosis in human breast cancer cells and the underlying molecular mechanism of this anti cancer effect. The treatment of MCF‑7 cells with rhamnetin was able to significantly inhibit cell proliferation and induce caspase‑3/9 activity in a dose‑ and time‑dependent manner, compared with untreated cells. In addition, treatment with rhamnetin was able to significantly promote the expression of p53 protein and microRNA (miR‑)34a compared with untreated cells. The treatment with rhamnetin also suppressed the expression of Notch1 protein in MCF‑7 cells compared with untreated cells. Subsequently, miR‑24a expression was promoted in rhamnetin‑treated MCF‑7 cells using a miR‑34a plasmid. The overexpression of miR‑34a was able to significantly inhibit cell viability and induce caspase‑3/9 activity in MCF‑7 cells following treatment with rhamnetin. Furthermore, the overexpression of miR‑34a was able to significantly promote the expression of p53 protein and miR‑34a, and suppress the expression of Notch1 protein in rhamnetin‑treated MCF‑7 cells. Therefore, the results of the present study demonstrated that rhamnetin induced apoptosis in human breast cancer cells via the miR‑34a/Notch‑1 signaling pathway.
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January-2019
Volume 17 Issue 1

Print ISSN: 1792-1074
Online ISSN:1792-1082

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Spandidos Publications style
Lan L, Wang Y, Pan Z, Wang B, Yue Z, Jiang Z, Li L, Wang C and Tang H: Rhamnetin induces apoptosis in human breast cancer cells via the miR‑34a/Notch‑1 signaling pathway. Oncol Lett 17: 676-682, 2019
APA
Lan, L., Wang, Y., Pan, Z., Wang, B., Yue, Z., Jiang, Z. ... Tang, H. (2019). Rhamnetin induces apoptosis in human breast cancer cells via the miR‑34a/Notch‑1 signaling pathway. Oncology Letters, 17, 676-682. https://doi.org/10.3892/ol.2018.9575
MLA
Lan, L., Wang, Y., Pan, Z., Wang, B., Yue, Z., Jiang, Z., Li, L., Wang, C., Tang, H."Rhamnetin induces apoptosis in human breast cancer cells via the miR‑34a/Notch‑1 signaling pathway". Oncology Letters 17.1 (2019): 676-682.
Chicago
Lan, L., Wang, Y., Pan, Z., Wang, B., Yue, Z., Jiang, Z., Li, L., Wang, C., Tang, H."Rhamnetin induces apoptosis in human breast cancer cells via the miR‑34a/Notch‑1 signaling pathway". Oncology Letters 17, no. 1 (2019): 676-682. https://doi.org/10.3892/ol.2018.9575