miR‑34a inhibits esophageal squamous cell carcinoma progression via regulation of FOXM1

Zhou,Haibo; Yang,Li; Xu,Xinhua; Lu,Mingqian; Guo,Rong; Li,Daojun; Huang,Qiao; Liu,Yang; Deng,Glenn; Xu,Yalin

doi:10.3892/ol.2018.9593

miR‑34a inhibits esophageal squamous cell carcinoma progression via regulation of FOXM1

Authors:
- Haibo Zhou
- Li Yang
- Xinhua Xu
- Mingqian Lu
- Rong Guo
- Daojun Li
- Qiao Huang
- Yang Liu
- Glenn Deng
- Yalin Xu
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Affiliations: Institute of Oncology, The First College of Clinical Medical Science, Yichang Central People's Hospital Affiliated to China Three Gorges University, Yichang, Hubei 443000, P.R. China, Department of Thyroid and Breast, The First College of Clinical Medical Science, Yichang Central People's Hospital Affiliated to China Three Gorges University, Yichang, Hubei 443000, P.R. China, School of Medicine, Stanford University, Stanford, CA 94305, USA, Department of Clinical Laboratory, Chibi Pu Spinning Hospital, Chibi, Hubei 437321, P.R. China
Published online on: October 17, 2018 https://doi.org/10.3892/ol.2018.9593
Pages: 706-712

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Abstract

Downregulation of microRNA‑34a (miR‑34a) has frequently been observed in esophageal squamous cell carcinoma (ESCC). However, the underlying role and molecular mechanism of miR‑34a in ESCC remains largely unknown. In the current study, it was demonstrated that miR‑34a was downregulated and forkhead box M1 (FOXM1), a target gene of miR‑34a, was upregulated in ESCC tumor tissues. Overexpression of miR‑34a decreased FOXM1 mRNA and protein expression in the ESCC cell lines tested (TE‑1 and TE‑8). Inhibition of miR‑34a increased FOXM1 mRNA and protein levels in human esophageal epithelial cells (HEEC). In addition, miR‑34a mimics reduced the relative luciferase activity of ESCC cells transfected with FOXM1 3'UTR‑WT, but not FOXM1 3'UTR‑Mut. The CCK8 assay and scratch wound healing assay showed that overexpression of miR‑34a induced inhibition of cell proliferation and cell migration. Additionally, transfection with miR‑34a mimics reduced the expression of key genes involved in cell migration (MMP2 and MMP9) in ESCC cells. Thus, the present data demonstrated that miR‑34a suppressed ESCC progression by directly targeting FOXM1.

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