Sirtuin‑7 knockdown inhibits the growth of endometrial cancer cells by inducing apoptosis via the NF‑κB signaling pathway

Mao,Shiqin; Ma,Jimin; Yu,Hong

doi:10.3892/ol.2018.9698

Sirtuin‑7 knockdown inhibits the growth of endometrial cancer cells by inducing apoptosis via the NF‑κB signaling pathway

Authors:
- Shiqin Mao
- Jimin Ma
- Hong Yu
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Affiliations: Department of Gynaecology, People's Hospital of Jingjiang, Jingjiang, Jiangsu 214500, P.R. China, Department of Gynaecology, The Third People's Hospital of Liaocheng, Liaocheng, Shandong 252000, P.R. China, Department of Critical Care Medicine, Harbin Second Hospital, Harbin, Heilongjiang 150036, P.R. China
Published online on: November 14, 2018 https://doi.org/10.3892/ol.2018.9698
Pages: 937-943
Copyright: © Mao et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Sirtuin‑7 is an evolutionarily conserved NAD‑dependent deacetylase, which serves an important role in carcinogenesis. However, the potential mechanism of sirtuin‑7 in endometrial cancer has not yet been investigated. The purpose of the present study was to investigate whether sirtuin‑7 exhibits inhibitory effects on endometrial cancer cells. The potential mechanisms mediated by sirtuin‑7 in endometrial cancer cells were also investigated. The expression levels of sirtuin‑7 in endometrial cancer cells were compared with normal endometrial cells using western blotting. The results demonstrated that sirtuin‑7 is overexpressed in endometrial cancer cells compared with normal endometrial cells. The downregulation of sirtuin‑7 inhibited the growth and invasiveness of endometrial cancer cells. The knockdown of sirtuin‑7 was observed to increase the sensitivity of the endometrial cancer cells to cisplatin treatment in vitro. An investigation into the potential molecular mechanism demonstrated that sirtuin‑7 knockdown promoted the apoptosis of endometrial cancer cells by regulating the nuclear factor (NF)‑κB signaling pathway. The knockdown of sirtuin‑7 inhibited NF‑κB expression and resulted in a decrease in the expression of NF‑κB target proteins that are anti‑apoptotic: Bcl‑xl, Bcl‑2 and Mcl‑1. Sirtuin‑7 knockdown also resulted in an increase of the NF‑κB target proteins that are pro‑apoptotic: Caspase‑3, Bad and Bax. In conclusion, the present study demonstrated that sirtuin‑7 knockdown was able to markedly inhibit the growth of endometrial cancer cells, suggesting that sirtuin‑7 may be a potential therapeutic target for endometrial cancer therapy.

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