Decreased expression of ZO‑1 is associated with tumor metastases in liver cancer

  • Authors:
    • Xueli Zhang
    • Liang Wang
    • Haitao Zhang
    • Fang Tu
    • Yong Qiang
    • Cuifang Nie
  • View Affiliations

  • Published online on: November 27, 2018     https://doi.org/10.3892/ol.2018.9765
  • Pages: 1859-1864
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Abstract

Certain types of cancer exhibit downregulated expression of zonula occludens-1 (ZO‑1), which serves an important function in tumor progression; however, the underlying molecular mechanisms that lead to this downregulation in cancer remain unclear. In the present study, the expression of ZO‑1 in liver cancer (LC) tissues was investigated. Western blot and reverse transcription‑quantitative polymerase chain reaction assays were used to detect the expression of ZO‑1 protein and mRNA in LC tissues and paired adjacent non‑tumorous tissues. The results indicated that, compared with non‑tumorous tissues, the expression of ZO‑1 was significantly downregulated at the protein (P<0.001) and mRNA (P=0.006) levels in LC tissue samples. In addition, various cellular and molecular methods were applied, including MTT, colony formation, flow cytometry and Transwell assays. The results indicated that overexpression of ZO‑1 inhibited cell viability, proliferation and migration, and induced G0/G1 phase arrest in vitro.
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February-2019
Volume 17 Issue 2

Print ISSN: 1792-1074
Online ISSN:1792-1082

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Spandidos Publications style
Zhang X, Wang L, Zhang H, Tu F, Qiang Y and Nie C: Decreased expression of ZO‑1 is associated with tumor metastases in liver cancer. Oncol Lett 17: 1859-1864, 2019
APA
Zhang, X., Wang, L., Zhang, H., Tu, F., Qiang, Y., & Nie, C. (2019). Decreased expression of ZO‑1 is associated with tumor metastases in liver cancer. Oncology Letters, 17, 1859-1864. https://doi.org/10.3892/ol.2018.9765
MLA
Zhang, X., Wang, L., Zhang, H., Tu, F., Qiang, Y., Nie, C."Decreased expression of ZO‑1 is associated with tumor metastases in liver cancer". Oncology Letters 17.2 (2019): 1859-1864.
Chicago
Zhang, X., Wang, L., Zhang, H., Tu, F., Qiang, Y., Nie, C."Decreased expression of ZO‑1 is associated with tumor metastases in liver cancer". Oncology Letters 17, no. 2 (2019): 1859-1864. https://doi.org/10.3892/ol.2018.9765