M3, a natural lignan xyloside, exhibits potent anticancer activity in HCT116 cells

Shen,Weidong; Zhao,Yu; Chen,Haijiao; Zhang,Ting; Wu,Shenbao; Liu,Pengfei

doi:10.3892/ol.2018.9823

M3, a natural lignan xyloside, exhibits potent anticancer activity in HCT116 cells

Authors:
- Weidong Shen
- Yu Zhao
- Haijiao Chen
- Ting Zhang
- Shenbao Wu
- Pengfei Liu
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Affiliations: Department of Gastroenterology, The Affiliated Jiangyin Hospital of Nantong University, Jiangyin, Jiangsu 214400, P.R. China, School of Pharmacy, Nantong University, Nantong, Jiangsu 226019, P.R. China, Department of Center Laboratory, The Affiliated Jiangyin Hospital of Nantong University, Jiangyin, Jiangsu 214400, P.R. China, Department of Gastroenterology, The Affiliated Yiwu Hospital of Wenzhou Medical College, Yiwu, Zhejiang 322000, P.R. China
Published online on: December 12, 2018 https://doi.org/10.3892/ol.2018.9823
Pages: 2117-2122
Copyright: © Shen et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

4‑O‑(2',3',4'‑tri‑O‑methyl‑β‑D‑xylopyranosyl) diphyllin (M3) is a cytotoxic compound that was first isolated from the aerial parts of Phyllanthus taxodiifolius. The current study demonstrated that M3, a lignan xyloside, exhibits anticancer effects in a number of cancer cell lines by MTT test, including HCT116 cells. An association was identified between M3 treatment and the reduced proliferation of cancer cells; the half maximal inhibitory concentration (IC50) value of M3 ranged from 0.08 to 1.12 µM. Furthermore, M3 was revealed to exhibit a stronger antiproliferative effect by Annexin‑V‑FLUOS test compared with VP‑16, another natural lignan used in cancer treatment. Notably, the IC50 value of M3 with MTT test in HCT116 cells was 0.08 µM. In addition, it was revealed that M3 could induce apoptosis in HCT116 cells in a caspase‑3‑dependent manner at a lower concentration compared with VP‑16. Further analysis identified that the antiproliferation effect of M3 was associated with the promotion of microtubule depolymerization by CytoDYNAMIX Screen 03 Tubulin Polymerization assay. In summary, the current study demonstrated that M3 could inhibit proliferation and induce apoptosis in HCT116 cells, which supports a potential therapeutic application of M3 in cancer treatment, particularly in colon cancer.

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