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Addition of free poloxamer 407 to a new gene vector P407‑PEI‑K12 solution forms a sustained‑release in situ hypergel that enhances cell transfection and extends gene expression

  • Authors:
    • Hongmei Shu
    • Yaguang Zhang
    • Min Zhang
    • Junwen Wu
    • Mingxiao Cui
    • Kehai Liu
    • Jun Wang

  • View Affiliations

  • Published online on: January 17, 2019     https://doi.org/10.3892/ol.2019.9944
  • Pages: 3085-3096

  • Copyright: © Shu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

To address the concern around the efficiency/cytotoxicity ratio and the tumor‑targeting effects of polyethylenimine (PEI), is a non‑viral gene vector used for the delivery of the cancer therapy gene, poloxamer 407 (P407)‑PEI‑K12, was synthesized by cross‑linking low‑molecular weight PEI with P407 and further coupling a bifunctional peptide, K12, which is comprised of the tumor‑targeting peptide tLyP‑1 and the nuclear localization sequence. Furthermore, the addition of free P407 into the polymer/DNA complex solution produced a temperature‑sensitive in situ gel‑P407/P407‑PEI‑K12/DNA complex, which improved the effects of sustained‑release gene delivery and transfection efficiency. The specificity, cytotoxicity and gene transfection efficiency of P407‑PEI‑K12 was investigated in Hela cells in vitro. The polymer efficiently prevented the degradation of plasmid DNA by DNase I and had a marked ability for serum tolerance. Agarose gel electrophoresis revealed that plasmid DNA was efficiently condensed and protected. The higher transfection efficiency of P407‑PEI‑K12h (the molar ratio of P407‑PEI and K12 is 1:10) was achieved with a polymer and plasmid DNA ratio (w/w) of 20:1. The ability of free P407 to promote the transfection of the polymer/DNA complex was high (0.09%). The half‑life of the P407/P407‑PEI‑K12‑h/DNA gel complex was 228 min, and the transfection efficiency of the P407/P407‑PEI‑K12‑h/DNA complex was markedly higher compared to that of the P407‑PEI‑K12‑h/DNA complex at various release times.
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March-2019
Volume 17 Issue 3

Print ISSN: 1792-1074
Online ISSN:1792-1082

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Spandidos Publications style
Shu H, Zhang Y, Zhang M, Wu J, Cui M, Liu K and Wang J: Addition of free poloxamer 407 to a new gene vector P407‑PEI‑K12 solution forms a sustained‑release in situ hypergel that enhances cell transfection and extends gene expression. Oncol Lett 17: 3085-3096, 2019
APA
Shu, H., Zhang, Y., Zhang, M., Wu, J., Cui, M., Liu, K., & Wang, J. (2019). Addition of free poloxamer 407 to a new gene vector P407‑PEI‑K12 solution forms a sustained‑release in situ hypergel that enhances cell transfection and extends gene expression. Oncology Letters, 17, 3085-3096. https://doi.org/10.3892/ol.2019.9944
MLA
Shu, H., Zhang, Y., Zhang, M., Wu, J., Cui, M., Liu, K., Wang, J."Addition of free poloxamer 407 to a new gene vector P407‑PEI‑K12 solution forms a sustained‑release in situ hypergel that enhances cell transfection and extends gene expression". Oncology Letters 17.3 (2019): 3085-3096.
Chicago
Shu, H., Zhang, Y., Zhang, M., Wu, J., Cui, M., Liu, K., Wang, J."Addition of free poloxamer 407 to a new gene vector P407‑PEI‑K12 solution forms a sustained‑release in situ hypergel that enhances cell transfection and extends gene expression". Oncology Letters 17, no. 3 (2019): 3085-3096. https://doi.org/10.3892/ol.2019.9944