Correlation of miR-425-5p and IL-23 with pancreatic cancer

Lu,Yanmin; Wu,Xinqing; Wang,Jinxiang

doi:10.3892/ol.2019.10099

Correlation of miR-425-5p and IL-23 with pancreatic cancer

Authors:
- Yanmin Lu
- Xinqing Wu
- Jinxiang Wang
View Affiliations

Affiliations: Department of Radiotherapy, Traditional Chinese Medical Hospital of Huangdao District, Qingdao, Shandong 266555, P.R. China, Department of Clinical Laboratory, Shouguang Hospital of Traditional Chinese Medicine, Shouguang, Shandong 262700, P.R. China, Department of Radiotherapy, Weifang Medical University Hospital, Weifang, Shandong 261031, P.R. China
Published online on: March 4, 2019 https://doi.org/10.3892/ol.2019.10099
Pages: 4595-4599
Copyright: © Lu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )

Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )

Cited By (CrossRef): 0 citations Loading Articles...

Abstract

The aim of this study was to detect the levels of miR-425-5p and IL-23 in tissues of pancreatic cancer patients and to explore their effects on pancreatic cancer. A retrospective analysis of 76 cases of pancreatic cancer tissue specimens and normal fresh tissue specimens at 50 mm adjacent to the corresponding cancer surgically resected in the first diagnosis was performed, in the Department of Radiotherapy of Traditional Chinese Medical Hospital of Huangdao District and Weifang Medical University Hospital from October 2015 to October 2016. RT-qPCR was used to detect miR-425-5p levels in pancreatic cancer and adjacent tissues, and ELISA to detect IL-23 levels in the tissues. The correlation of miR-425-5p and IL-23 in pancreatic cancer tissues with clinicopathological parameters was analyzed. The expression levels of miR-425-5p and IL-23 were significantly higher in pancreatic cancer tissues than those in adjacent tissues, with statistically significant difference (P<0.001). The expression level of miR-425-5p was positively correlated with IL-23 in pancreatic cancer tissues (r=0.432, P<0.001), and of miR-425-5p in tumor tissues of pancreatic cancer patients was correlated with lymph node metastasis, clinical stage and differentiation degree (P<0.001). That of IL-23 in tumor tissues of pancreatic cancer patients was correlated with clinical stage (P<0.001). The expression levels of miR-425-5p and IL-23 in tissues of pancreatic cancer patients are higher than those in adjacent tissues. The expression level of miR-425-5p is positively correlated with that of IL-23 in pancreatic cancer tissues, and that of miR-425-5p in tumor tissues of pancreatic cancer patients was correlated with lymph node metastasis, clinical stage and differentiation degree. IL-23 in tumor tissues of pancreatic cancer patients was correlated with clinical stage. miR-425-5p and IL-23 may be involved in the pathological development of pancreatic cancer.

View Figures

View References

1	Li D, Xie K, Wolff R and Abbruzzese JL: Pancreatic cancer. Lancet. 363:1049–1057. 2004. View Article : Google Scholar : PubMed/NCBI
2	Vincent A, Herman J, Schulick R, Hruban RH and Goggins M: Pancreatic cancer. Lancet. 378:607–620. 2011. View Article : Google Scholar : PubMed/NCBI
3	Rochefort MM, Ankeny JS, Kadera BE, Donald GW, Isacoff W, Wainberg ZA, Hines OJ, Donahue TR, Reber HA and Tomlinson JS: Impact of tumor grade on pancreatic cancer prognosis: Validation of a novel TNMG staging system. Ann Surg Oncol. 20:4322–4329. 2013. View Article : Google Scholar : PubMed/NCBI
4	Koliopanos A, Avgerinos C, Farfaras A, Manes C and Dervenis C: Radical resection of pancreatic cancer. Hepatobiliary Pancreat Dis Int. 7:11–18. 2008.PubMed/NCBI
5	Abe M, Kondo S, Hirano S, Ambo Y, Tanaka E, Morikawa T, Okushiba S and Katoh H: Long-term survival after radical resection of advanced pancreatic cancer: A case report with special reference to CD8⁺ T-cell infiltration. Int J Gastrointest Cancer. 33:107–110. 2003. View Article : Google Scholar : PubMed/NCBI
6	Lowenfels AB and Maisonneuve P: Epidemiology and risk factors for pancreatic cancer. Best Pract Res Clin Gastroenterol. 20:197–209. 2006. View Article : Google Scholar : PubMed/NCBI
7	Kashiwagi Y, Kato N, Sassa T, Nishitsuka K, Yamamoto T, Takamura H and Yamashita H: Cotylenin A inhibits cell proliferation and induces apoptosis and PAX6 mRNA transcripts in retinoblastoma cell lines. Mol Vis. 16:970–982. 2010.PubMed/NCBI
8	Tchernitsa O, Kasajima A, Schäfer R, Kuban RJ, Ungethüm U, Györffy B, Neumann U, Simon E, Weichert W, Ebert MP, et al: Systematic evaluation of the miRNA-ome and its downstream effects on mRNA expression identifies gastric cancer progression. J Pathol. 222:310–319. 2010. View Article : Google Scholar : PubMed/NCBI
9	He B, Li T, Guan L, Liu FE, Chen XM, Zhao J, Lin S, Liu ZZ and Zhang HQ: CTNNA3 is a tumor suppressor in hepatocellular carcinomas and is inhibited by miR-425. Oncotarget. 7:8078–8089. 2016.PubMed/NCBI
10	Li L, Chen HZ, Chen FF, Li F, Wang M, Wang L, Li YQ and Gao DS: Global microRNA expression profiling reveals differential expression of target genes in 6-hydroxydopamine-injured MN9D cells. Neuromolecular Med. 15:593–604. 2013. View Article : Google Scholar : PubMed/NCBI
11	Yu J, Li A, Hong SM, Hruban RH and Goggins M: MicroRNA alterations of pancreatic intraepithelial neoplasias. Clin Cancer Res. 18:981–992. 2012. View Article : Google Scholar : PubMed/NCBI
12	Ding C, Xu J and Li J: ABT-874, a fully human monoclonal anti-IL-12/IL-23 antibody for the potential treatment of autoimmune diseases. Curr Opin Investig Drugs. 9:515–522. 2008.PubMed/NCBI
13	Langowski JL, Zhang X, Wu L, Mattson JD, Chen T, Smith K, Basham B, McClanahan T, Kastelein RA and Oft M: IL-23 promotes tumour incidence and growth. Nature. 442:461–465. 2006. View Article : Google Scholar : PubMed/NCBI
14	Livak KJ and Schmittgen TD: Analysis of relative gene expression data using realtime quantitative PCR and the 2(-Delta Delta C(T)) method. Methods. 25:402–408. 2001. View Article : Google Scholar : PubMed/NCBI
15	Siegel R, Naishadham D and Jemal A: Cancer statistics, 2013. CA Cancer J Clin. 63:11–30. 2013. View Article : Google Scholar : PubMed/NCBI
16	Zhang Z, Wen M, Guo J, Shi J, Wang Z, Tan B, Zhang G, Zheng X and Zhang A: Clinical value of miR-425-5p detection and its association with cell proliferation and apoptosis of gastric cancer. Pathol Res Pract. 213:929–937. 2017. View Article : Google Scholar : PubMed/NCBI
17	Ciric B, El-behi M, Cabrera R, Zhang GX and Rostami A: IL-23 drives pathogenic IL-17-producing CD8⁺ T cells. J Immunol. 182:5296–5305. 2009. View Article : Google Scholar : PubMed/NCBI
18	Shime H, Yabu M, Akazawa T, Kodama K, Matsumoto M, Seya T and Inoue N: Tumor-secreted lactic acid promotes IL-23/IL-17 proinflammatory pathway. J Immunol. 180:7175–7183. 2008. View Article : Google Scholar : PubMed/NCBI
19	Chen G, Liang Y, Guan X, Chen H, Liu Q, Lin B, Chen C, Huang M, Chen J, Wu W, et al: Circulating low IL-23: IL-35 cytokine ratio promotes progression associated with poor prognosisin breast cancer. Am J Transl Res. 8:2255–2264. 2016.PubMed/NCBI
20	Khodadadi A, Razmkhah M, Eskandari AR, Hosseini A, Habibagahi M, Ghaderi A and Jaberipour M: IL-23/IL-27 ratio in peripheral blood of patients with breast cancer. Iran J Med Sci. 39:350–356. 2014.PubMed/NCBI
21	Sun L, Jiang R, Li J, Wang B, Ma C, Lv Y and Mu N: MicoRNA-425-5p is a potential prognostic biomarker for cervical cancer. Ann Clin Biochem. 54:127–133. 2017. View Article : Google Scholar : PubMed/NCBI
22	Petanidis S, Anestakis D, Argyraki M, Hadzopoulou-Cladaras M and Salifoglou A: Differential expression of IL-17, 22 and 23 in the progression of colorectal cancer in patients with K-ras mutation: Ras signal inhibition and crosstalk with GM-CSF and IFN-γ. PLoS One. 8:e736162013. View Article : Google Scholar : PubMed/NCBI
23	Qian X, Gu L, Ning H, Zhang Y, Hsueh EC, Fu M, Hu X, Wei L, Hoft DF and Liu J: Increased Th17 cells in the tumor microenvironment is mediated by IL-23 via tumor-secreted prostaglandin E2. J Immunol. 190:5894–5902. 2013. View Article : Google Scholar : PubMed/NCBI